SEH1/YGL100W Summary Help

Standard Name SEH1 1
Systematic Name YGL100W
Feature Type ORF, Verified
Description Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 (1, 2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description SEc13 Homolog 1
Chromosomal Location
ChrVII:313234 to 314283 | ORF Map | GBrowse
Gene Ontology Annotations All SEH1 GO evidence and references
  View Computational GO annotations for SEH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
306 total interaction(s) for 214 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 95
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 17
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-purification: 4
  • Reconstituted Complex: 9

Genetic Interactions
  • Negative Genetic: 133
  • Positive Genetic: 38
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 349
Molecular Weight (Da) 39,122
Isoelectric Point (pI) 5.32
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:313234 to 314283 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1050 313234..314283 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003068

SEH1 encodes a nuclear pore protein (1, 8). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 8, 9, 10, 11). The structure of the vertebrate NPC has been studied extensively; recent reviews include 12, 13, 14, and , 15). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 8, 9, 16, 17, 18). Seh1p is part of subcomplex within the NPC that also includes Sec13p, Nup84p, Nup85p, and Nup120p (1, 8). Seh1p is tightly associated with Nup85p in the subcomplex (1). The seh1 null mutant is viable but grows slowly, especially at low temperatures (1). Deletion of both SEH1 and NUP84 is lethal (1).

Last updated: 1999-07-28 Contact SGD

References cited on this page View Complete Literature Guide for SEH1
1) Siniossoglou S, et al.  (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84(2):265-75
2) Lillo JA, et al.  (2000) Disruption and phenotypic analysis of six open reading frames from the left arm of Saccharomyces cerevisiae chromosome VII. Yeast 16(4):365-75
3) Siniossoglou S, et al.  (2000) Structure and assembly of the Nup84p complex. J Cell Biol 149(1):41-54
4) Lutzmann M, et al.  (2002) Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. EMBO J 21(3):387-97
5) Fernandez-Martinez J and Rout MP  (2009) Nuclear pore complex biogenesis. Curr Opin Cell Biol 21(4):603-12
6) Dokudovskaya S, et al.  (2011) A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics 10(6):M110.006478
7) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
8) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
9) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
10) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
11) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
12) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
13) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
14) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
15) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
16) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
17) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
18) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407