GCN2/YDR283C Summary Help

Standard Name GCN2 1
Systematic Name YDR283C
Alias AAS1 2 , NDR2 3 , AAS102 3
Feature Type ORF, Verified
Description Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control (4, 5, 6, 7)
Name Description General Control Nonderepressible 1
Chromosomal Location
ChrIV:1030049 to 1025070 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 173 cM
Gene Ontology Annotations All GCN2 GO evidence and references
  View Computational GO annotations for GCN2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
314 total interaction(s) for 188 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 41
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 11
  • Biochemical Activity: 4
  • Co-fractionation: 5
  • Reconstituted Complex: 26
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 19
  • Negative Genetic: 128
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 10
  • Positive Genetic: 32
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 1
  • Synthetic Rescue: 13

Expression Summary
Length (a.a.) 1,659
Molecular Weight (Da) 190,192
Isoelectric Point (pI) 6.31
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1030049 to 1025070 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 173 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4980 1030049..1025070 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002691
References cited on this page View Complete Literature Guide for GCN2
1) Lucchini G, et al.  (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33
2) Hinnebusch AG and Fink GR  (1983) Positive regulation in the general amino acid control of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 80(17):5374-8
3) Thireos G, et al.  (1984) 5' untranslated sequences are required for the translational control of a yeast regulatory gene. Proc Natl Acad Sci U S A 81(16):5096-100
4) Kubota H, et al.  (2001) Budding yeast GCN1 binds the GI domain to activate the eIF2alpha kinase GCN2. J Biol Chem 276(20):17591-6
5) Garcia-Barrio M, et al.  (2000) Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation. EMBO J 19(8):1887-99
6) Hinnebusch AG and Natarajan K  (2002) Gcn4p, a master regulator of gene expression, is controlled at multiple levels by diverse signals of starvation and stress. Eukaryot Cell 1(1):22-32
7) Menacho-Marquez M, et al.  (2007) Gcn2p regulates a G1/S cell cycle checkpoint in response to DNA damage. Cell Cycle 6(18):2302-5