GCS1/YDL226C Summary Help

Standard Name GCS1 1
Systematic Name YDL226C
Feature Type ORF, Verified
Description ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication (2, 3, 4, 5 and see Summary Paragraph)
Name Description Growth Cold Sensitive 6
Chromosomal Location
ChrIV:52173 to 51115 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All GCS1 GO evidence and references
  View Computational GO annotations for GCS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
265 total interaction(s) for 148 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • Co-purification: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Rescue: 18
  • Negative Genetic: 82
  • Phenotypic Enhancement: 30
  • Phenotypic Suppression: 13
  • Positive Genetic: 21
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 40
  • Synthetic Rescue: 11

Expression Summary
Length (a.a.) 352
Molecular Weight (Da) 39,296
Isoelectric Point (pI) 5.78
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:52173 to 51115 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1059 52173..51115 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002385

GCS1 encodes a GTPase activating protein (GAP) for ADP ribosylation factors (ARFs, encoded by ARF1, ARF2, and ARF3 in yeast), and interacts genetically with ARF1 and ARF2 (7). ARFs are GTPases of the Ras superfamily that regulate the formation of coated vesicles in intracellular trafficking (8, 9). The process of vesicle formation in the exocytic and endocytic pathways has been recently reviewed (10), as has the role of ARF (11).

GCS1 was originally identified as a gene required for cells to reenter the mitotic cell cycle from stationary phase (6, 1). Gcs1p binds actin in vitro, and may interact with the cytoskeleton in vivo. Disruption of GCS1 causes cytoskeletal defects, and GCS1 and shows genetic interactions with SLA2 and SAC6, which encode actin binding proteins (12). A second ARF GAP, Glo3p, has been identified in yeast; a gcs1 glo3 double deletion is inviable (13). Genetic interactions with BET1, BOS1, and SEC22, which encode v-SNARES, implicate Gcs1p and Glo3p in transport between the ER and the Golgi (13). A protein with sequence similarity to Gcs1p, centaurin alpha, has been found in rat and human (14).

Last updated: 1999-10-29 Contact SGD

References cited on this page View Complete Literature Guide for GCS1
1) Ireland LS, et al.  (1994) A member of a novel family of yeast 'zn-finger' proteins mediates the transition from stationary phase to cell proliferation. EMBO J 13(16):3812-21
2) Poon PP, et al.  (2001) Expression, analysis, and properties of yeast ADP-ribosylation factor (ARF) GTPase activating proteins (GAPs) Gcs1 and Glo3. Methods Enzymol 329:317-24
3) Poon PP, et al.  (2001) The Gcs1 and Age2 ArfGAP proteins provide overlapping essential function for transport from the yeast trans-Golgi network. J Cell Biol 155(7):1239-50
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Connolly JE and Engebrecht J  (2006) The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p. Eukaryot Cell 5(1):112-24
6) Drebot MA, et al.  (1987) A yeast mutant conditionally defective only for reentry into the mitotic cell cycle from stationary phase. Proc Natl Acad Sci U S A 84(22):7948-52
7) Poon PP, et al.  (1996) Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase-activating protein. Proc Natl Acad Sci U S A 93(19):10074-7
8) Moss J and Vaughan M  (1998) Molecules in the ARF orbit. J Biol Chem 273(34):21431-4
9) Moss J and Vaughan M  (1999) Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem 193(1-2):153-7
10) Springer S, et al.  (1999) A primer on vesicle budding. Cell 97(2):145-8
11) Roth MG  (1999) Snapshots of ARF1: implications for mechanisms of activation and inactivation. Cell 97(2):149-52
12) Blader IJ, et al.  (1999) GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro. Mol Biol Cell 10(3):581-96
13) Poon PP, et al.  (1999) Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function. EMBO J 18(3):555-64
14) Venkateswarlu K, et al.  (1999) Identification of centaurin-alpha1 as a potential in vivo phosphatidylinositol 3,4,5-trisphosphate-binding protein that is functionally homologous to the yeast ADP-ribosylation factor (ARF) GTPase-activating protein, Gcs1. Biochem J 340 ( Pt 2)():359-63