MYO3/YKL129C Summary Help

Standard Name MYO3 1
Systematic Name YKL129C
Feature Type ORF, Verified
Description One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication (2, 3, 4, 5 and see Summary Paragraph)
Name Description MYOsin 1
Chromosomal Location
ChrXI:200167 to 196349 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MYO3 GO evidence and references
  View Computational GO annotations for MYO3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
130 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 12
  • Biochemical Activity: 3
  • Protein-peptide: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 48

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 12
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 8
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,272
Molecular Weight (Da) 142,451
Isoelectric Point (pI) 9.89
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:200167 to 196349 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3819 200167..196349 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001612

MYO3 is a non-essential gene that encodes one of two class I myosins found in Saccharomyces cerevisiae (6). Both Myo3p and the other yeast class I myosin, Myo5p, localize to actin cortical patches (7, 8). Cortical patches are found at sites of polarized cell growth, and appear to be involved in endocytosis and cell wall biogenesis (9, 8). Deletion of either MYO3 or MYO5 has little effect on cell growth, but the myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization (1, , 2). In a myo3 null background, the temperature sensitive myo5-1 mutant has a defect in the internalization step of endocytosis (2). Class I myosins play several different roles in many other eukaryotes; see reference 10 for review.

Last updated: 2000-03-24 Contact SGD

References cited on this page View Complete Literature Guide for MYO3
1) Goodson HV and Spudich JA  (1995) Identification and molecular characterization of a yeast myosin I. Cell Motil Cytoskeleton 30(1):73-84
2) Geli MI and Riezman H  (1996) Role of type I myosins in receptor-mediated endocytosis in yeast. Science 272(5261):533-5
3) Anderson BL, et al.  (1998) The Src homology domain 3 (SH3) of a yeast type I myosin, Myo5p, binds to verprolin and is required for targeting to sites of actin polarization. J Cell Biol 141(6):1357-70
4) Tanaka K and Matsui Y  (2001) Functions of unconventional myosins in the yeast Saccharomyces cerevisiae. Cell Struct Funct 26(6):671-5
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Brown SS  (1997) Myosins in yeast. Curr Opin Cell Biol 9(1):44-8
7) Goodson HV, et al.  (1996) Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. J Cell Biol 133(6):1277-91
8) Pruyne D and Bretscher A  (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
9) Botstein D, et al.  (1997) "The yeast cytoskeleton." Pp. 1-90 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
10) Mooseker MS and Cheney RE  (1995) Unconventional myosins. Annu Rev Cell Dev Biol 11():633-75