PAN1/YIR006C Summary Help

Standard Name PAN1 1
Systematic Name YIR006C
Alias MDP3 , MIP3 , DIM2 2
Feature Type ORF, Verified
Description Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress; previously thought to be a subunit of poly(A) ribonuclease (2, 3, 4, 5, 6)
Name Description Poly(A)-binding protein-dependent poly(A) riboNuclease 1
Chromosomal Location
ChrIX:369908 to 365466 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PAN1 GO evidence and references
  View Computational GO annotations for PAN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
reduction of function
140 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 17
  • Biochemical Activity: 7
  • Co-purification: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 26

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 8
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 24
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 1,480
Molecular Weight (Da) 160,267
Isoelectric Point (pI) 5.02
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIX:369908 to 365466 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4443 369908..365466 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001445
References cited on this page View Complete Literature Guide for PAN1
1) Sachs AB and Deardorff JA  (1992) Translation initiation requires the PAB-dependent poly(A) ribonuclease in yeast. Cell 70(6):961-73
2) Wendland B, et al.  (1996) A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15. J Cell Biol 135(6 Pt 1):1485-500
3) Tang HY and Cai M  (1996) The EH-domain-containing protein Pan1 is required for normal organization of the actin cytoskeleton in Saccharomyces cerevisiae. Mol Cell Biol 16(9):4897-914
4) Zeng G, et al.  (2001) Regulation of yeast actin cytoskeleton-regulatory complex Pan1p/Sla1p/End3p by serine/threonine kinase Prk1p. Mol Biol Cell 12(12):3759-72
5) Duncan MC, et al.  (2001) Yeast Eps15-like endocytic protein, Pan1p, activates the Arp2/3 complex. Nat Cell Biol 3(7):687-90
6) Reiter W, et al.  (2012) Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins. Proteomics 12(19-20):3030-43