NUP159/YIL115C Summary Help

Standard Name NUP159 1
Systematic Name YIL115C
Alias NUP158 , RAT7 2
Feature Type ORF, Verified
Description FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 and see Summary Paragraph)
Name Description NUclear Pore 14
Chromosomal Location
ChrIX:148709 to 144327 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All NUP159 GO evidence and references
  View Computational GO annotations for NUP159
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
172 total interaction(s) for 89 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 61
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 21
  • Co-crystal Structure: 2
  • Co-purification: 2
  • PCA: 4
  • Protein-peptide: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 10
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Positive Genetic: 17
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 16
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 1,460
Molecular Weight (Da) 158,906
Isoelectric Point (pI) 4.56
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIX:148709 to 144327 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4383 148709..144327 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001377

NUP159 encodes an essential nuclear pore protein (2, 1, 15). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 15, 16, 17, 18). The structure of the vertebrate NPC has been studied extensively; recent reviews include 19, 20, 21, and 22. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (23, 24). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 15, 16, 25, 26, 27). Nup159p is one of a group of nucleoporins that contain multiple repeats of the amino acids GLFG (2, 1, 15), and is found only on the cytoplasmic face of the pore (1). At the restrictive temperature, a temperature-sensitive nup159 allele changes the distribution of NPCs within the nuclear envelope, and is defective in mRNA export from the nucleus (2). The phenotype can be partially suppressed by overexpression of the RNA export factor Gle1p (28, 15). Domain analysis suggests that the N-terminal thrid of Nup159p is important for its role in nuclear mRNA export (29). While nup159 mutants do not show protein import defects (15), Nup159p associates physically with Pse1p, a member of a protein family implicated in nuclear protein import (30). nup159 mutations are synthetically lethal with mutations in several other nucleoporin genes (15).

Last updated: 1999-08-12 Contact SGD

References cited on this page View Complete Literature Guide for NUP159
1) Kraemer DM, et al.  (1995) The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate. J Biol Chem 270(32):19017-21
2) Gorsch LC, et al.  (1995) A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129(4):939-55
3) Strasser K, et al.  (2000) Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export. J Cell Biol 150(4):695-706
4) Hodge CA, et al.  (1999) Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells. EMBO J 18(20):5778-88
5) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
6) Stage-Zimmermann T, et al.  (2000) Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell 11(11):3777-89
7) Bailer SM, et al.  (2000) Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex. J Biol Chem 275(31):23540-8
8) Grosshans H, et al.  (2001) Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export. J Cell Biol 153(4):745-62
9) Strawn LA, et al.  (2004) Minimal nuclear pore complexes define FG repeat domains essential for transport. Nat Cell Biol 6(3):197-206
10) Weirich CS, et al.  (2004) The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore. Mol Cell 16(5):749-60
11) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
12) Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
13) Noble KN, et al.  (2011) The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1. Genes Dev 25(10):1065-77
14) Davis LI and Fink GR  (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
15) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
16) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
17) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
18) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
19) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
20) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
21) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
22) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
23) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
24) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
25) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
26) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
27) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
28) Del Priore V, et al.  (1996) The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export. Mol Biol Cell 7(10):1601-21
29) Del Priore V, et al.  (1997) A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae. J Cell Sci 110 ( Pt 23):2987-99
30) Seedorf M, et al.  (1999) Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase. Mol Cell Biol 19(2):1547-57