SRB4/YER022W Summary Help

Standard Name SRB4 1
Systematic Name YER022W
Alias MED17 2
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination (1, 3, 4, 5)
Name Description Suppressor of RNA polymerase B 1
Chromosomal Location
ChrV:198812 to 200875 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SRB4 GO evidence and references
  View Computational GO annotations for SRB4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
conditional
Large-scale survey
null
overexpression
reduction of function
Resources
328 total interaction(s) for 100 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 128
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 67
  • Co-localization: 4
  • Co-purification: 50
  • PCA: 1
  • Reconstituted Complex: 14
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 22
  • Phenotypic Enhancement: 4
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3
  • Synthetic Rescue: 12

Resources
Expression Summary
histogram
Resources
Length (a.a.) 687
Molecular Weight (Da) 78,475
Isoelectric Point (pI) 5.08
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:198812 to 200875 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2064 198812..200875 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000824
References cited on this page View Complete Literature Guide for SRB4
1) Thompson CM, et al.  (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
4) Takagi Y and Kornberg RD  (2006) Mediator as a general transcription factor. J Biol Chem 281(1):80-9
5) Kaufmann R, et al.  (2010) Infantile Cerebral and Cerebellar Atrophy Is Associated with a Mutation in the MED17 Subunit of the Transcription Preinitiation Mediator Complex. Am J Hum Genet 87(5):667-70