ABP1/YCR088W Summary Help

Standard Name ABP1 1, 2
Systematic Name YCR088W
Feature Type ORF, Verified
Description Actin-binding protein of the cortical actin cytoskeleton; important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; inhibits barbed-end actin filament elongation; phosphorylation within its Proline-Rich Regio, mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences; mammalian homologue of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa) (1, 3, 4, 5, 6, 7)
Name Description Actin Binding Protein 2
Chromosomal Location
ChrIII:265068 to 266846 | ORF Map | GBrowse
Gbrowse
Genetic position: 80 cM
Gene Ontology Annotations All ABP1 GO evidence and references
  View Computational GO annotations for ABP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
174 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 29
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 12
  • Co-fractionation: 3
  • Co-localization: 4
  • FRET: 2
  • PCA: 2
  • Protein-peptide: 13
  • Reconstituted Complex: 2
  • Two-hybrid: 28

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 47
  • Phenotypic Enhancement: 2
  • Positive Genetic: 6
  • Synthetic Growth Defect: 6
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 11
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 592
Molecular Weight (Da) 65,576
Isoelectric Point (pI) 4.41
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:265068 to 266846 | ORF Map | GBrowse
SGD ORF map
Genetic position: 80 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1779 265068..266846 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000684
References cited on this page View Complete Literature Guide for ABP1
1) Drubin DG, et al.  (1990) Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I. Nature 343(6255):288-90
2) Drubin DG, et al.  (1988) Yeast actin-binding proteins: evidence for a role in morphogenesis. J Cell Biol 107(6 Pt 2):2551-61
3) Mulholland J, et al.  (1994) Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane. J Cell Biol 125(2):381-91
4) Olazabal IM and Machesky LM  (2001) Abp1p and cortactin, new "hand-holds" for actin. J Cell Biol 154(4):679-82
5) Garcia B, et al.  (2012) The importance of conserved features of yeast actin-binding protein 1 (Abp1p): the conditional nature of essentiality. Genetics 191(4):1199-211
6) Michelot A, et al.  (2013) Actin filament elongation in Arp2/3-derived networks is controlled by three distinct mechanisms. Dev Cell 24(2):182-95
7) Yang C, et al.  (2014) Regulation of cell survival by the HIP-55 signaling network. Mol Biosyst 10(6):1393-9