SRB6/YBR253W Summary Help

Standard Name SRB6 1
Systematic Name YBR253W
Alias MED22 2
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 3, 4)
Name Description Suppressor of RNA polymerase B 1
Chromosomal Location
ChrII:723270 to 723635 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SRB6 GO evidence and references
  View Computational GO annotations for SRB6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
repressible
unspecified
Resources
162 total interaction(s) for 55 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 109
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 18
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 8
  • PCA: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 121
Molecular Weight (Da) 13,863
Isoelectric Point (pI) 4.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:723270 to 723635 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..366 723270..723635 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000457
References cited on this page View Complete Literature Guide for SRB6
1) Thompson CM, et al.  (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
4) Takagi Y, et al.  (2006) Head module control of mediator interactions. Mol Cell 23(3):355-64