DAD3/YBR233W-A Summary Help

Standard Name DAD3 1
Systematic Name YBR233W-A
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis (2, 3, 4, 5, 6)
Name Description Duo1 And Dam1 interacting 1
Chromosomal Location
ChrII:684977 to 685261 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DAD3 GO evidence and references
  View Computational GO annotations for DAD3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Large-scale survey
null
Resources
22 total interaction(s) for 13 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Co-crystal Structure: 1
  • Co-purification: 7
  • PCA: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 94
Molecular Weight (Da) 10,848
Isoelectric Point (pI) 5.25
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:684977 to 685261 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2001-02-26
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..285 684977..685261 2011-02-03 2001-02-26
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000007595
References cited on this page View Complete Literature Guide for DAD3
1) Cheeseman IM, et al.  (2002) Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111(2):163-72
2) Hofmann C, et al.  (1998) Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function. J Cell Biol 143(4):1029-40
3) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
4) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
5) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
6) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9