AI4/Q0065 Summary

Standard Name	AI4
Systematic Name	Q0065
Feature Type	ORF, Verified
Description	Endonuclease I-SceII, encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity (1, 2)
Gene Product Alias	I-SceII

Chromosomal Location	ChrMito:13818 to 21935 \| ORF Map \| GBrowse

	View Computational GO annotations for AI4
Molecular Function
Manually curated	endonuclease activity (IDA)
Biological Process
Manually curated	intron homing (IMP) RNA splicing (IDA)
Cellular Component
Manually curated	mitochondrion (TAS)

	1 total interaction(s) for 1 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| CGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrMito:13818 to 21935 | |

This feature contains embedded feature(s): AI1 | AI2 | AI3

This feature is contained within: COX1

This feature is contained within: AI5_ALPHA

Last Update

Coordinates: 2000-05-19 | Sequence: 2000-05-19

Subfeature details

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000007264

1)	Wernette CM, et al. (1990) Purification of a site-specific endonuclease, I-Sce II, encoded by intron 4 alpha of the mitochondrial coxI gene of Saccharomyces cerevisiae. J Biol Chem 265(31):18976-82
2)	Dujardin G, et al. (1982) Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene. Nature 298(5875):628-32