Standard Name	DID2
Systematic Name	YKR035W-A
Alias	FTI1 , CHM1 , VPS46 1 , VPL30 1
Feature Type	ORF, Verified
Description	Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors (2, 3, 4)
Name Description	Doa4-Independent Degradation 2

Chromosomal Location	ChrXI:507939 to 508553 | ORF Map | GBrowse

	View Computational GO annotations for DID2
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	late endosome to vacuole transport (IMP) late endosome to vacuole transport via multivesicular body sorting pathway (IMP) protein targeting to vacuole (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) late endosome (IDA)

Classical genetics
null	tellurium atom accumulation: increased heat sensitivity: increased resistance to cadmium chloride: decreased resistance to tunicamycin: decreased resistance to mercaptoethanol: decreased resistance to L-1,4-dithiothreitol: decreased
reduction of function	GFP-Carboxypeptidase-S distribution: abnormal
Large-scale survey
null	anaerobic growth: decreased glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased haploinsufficient heat sensitivity: increased ionic stress resistance: decreased metal resistance: decreased resistance to dimethyl sulfoxide: decreased resistance to cycloheximide: decreased resistance to calcium dichloride: decreased resistance to 1,4-dithiothreitol: decreased resistance to ethanol: decreased resistance to sirolimus: decreased sporulation: decreased sporulation efficiency: decreased stress resistance: decreased vacuolar morphology: abnormal
overexpression	cell cycle progression: abnormal
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	110 total interaction(s) for 80 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-Western: 11 Co-crystal Structure: 1 Co-localization: 1 Far Western: 1 PCA: 4 Reconstituted Complex: 6 Two-hybrid: 28
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 34 Phenotypic Enhancement: 5 Phenotypic Suppression: 2 Positive Genetic: 2 Synthetic Growth Defect: 8 Synthetic Lethality: 1 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:507939 to 508553 | ORF Map | GBrowse
	This feature is contained within: OPI8
Last Update	Coordinates: 2011-02-03 | Sequence: 1999-07-17
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..615 507939..508553 2011-02-03 1999-07-17
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain CLIB382 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006435