MLC2/YPR188C Summary Help

MLC2 BASIC INFORMATION

Standard Name MLC2 1
Systematic Name YPR188C
Feature Type ORF, Verified
Description Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring (1 and see Summary Paragraph)
Name Description Myo1p Light Chain
GO Annotations All MLC2 GO evidence and references
    View Computational GO annotations for MLC2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All MLC2 Phenotype details and references
Large-scale survey
null
Interactions MLC2 All interactions details and references
14 total interaction(s) for 9 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Two-hybrid: 2
Genetic Interactions
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1
Sequence Information
ChrXVI:912475 to 911984 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-07-21 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..492 912475..911984 2004-07-21 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000006392

MLC2 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for MLC2

SUMMARY PARAGRAPH for MLC2

Mlc2p is a regulatory light chain (RLC) for Myo1p, a type II myosin, while Mlc1p is the essential light chain (ELC) for Myo1p. Mlc2p localizes to the bud neck through an interaction with the IQ2 domain of Myo1p. Deletion of MLC2 causes defect in the disassembly of the Myo1p ring at the end of ring contraction (1).

MLC2 displays significant sequence homology to calmodulin or myosin light chain related proteins. Like other light chains, Mlc2p contains an EF hand and a serine residue, both close to the N-terminus, and the serine corresponds to one of the activating phosphorylation sites in the human type II myosin RLC (1 and references therein). The family of RLCs for nonmuscle type II myosins share limited sequence homology and their function in modulating the heavy-chain activity depends on the organismal complexity. Mutations in the fly RLC gene, spaghetti-squash, cause lethality (1 and references therein), while deletion of MLC2 in S. cerevisiae produces a defect in the disassembly of the Myo1p ring.

Last updated: 2007-02-15

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MLC2]

1) Luo J, et al.  (2004) Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J Cell Biol 165(6):843-55