MLC2/YPR188C Summary Help

Standard Name MLC2 1
Systematic Name YPR188C
Feature Type ORF, Verified
Description Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring (1 and see Summary Paragraph)
Name Description Myo1p Light Chain
Chromosomal Location
ChrXVI:912479 to 911988 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MLC2 GO evidence and references
  View Computational GO annotations for MLC2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
28 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 7
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 163
Molecular Weight (Da) 18,520
Isoelectric Point (pI) 4.15
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:912479 to 911988 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..492 912479..911988 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006392
SUMMARY PARAGRAPH for MLC2

Mlc2p is a regulatory light chain (RLC) for Myo1p, a type II myosin, while Mlc1p is the essential light chain (ELC) for Myo1p. Mlc2p localizes to the bud neck through an interaction with the IQ2 domain of Myo1p. Deletion of MLC2 causes defect in the disassembly of the Myo1p ring at the end of ring contraction (1).

MLC2 displays significant sequence homology to calmodulin or myosin light chain related proteins. Like other light chains, Mlc2p contains an EF hand and a serine residue, both close to the N-terminus, and the serine corresponds to one of the activating phosphorylation sites in the human type II myosin RLC (1 and references therein). The family of RLCs for nonmuscle type II myosins share limited sequence homology and their function in modulating the heavy-chain activity depends on the organismal complexity. Mutations in the fly RLC gene, spaghetti-squash, cause lethality (1 and references therein), while deletion of MLC2 in S. cerevisiae produces a defect in the disassembly of the Myo1p ring.

Last updated: 2007-02-15 Contact SGD

References cited on this page View Complete Literature Guide for MLC2
1) Luo J, et al.  (2004) Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J Cell Biol 165(6):843-55