GDB1/YPR184W Summary Help

Standard Name GDB1
Systematic Name YPR184W
Feature Type ORF, Verified
Description Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress (1, 2, 3, 4 and see Summary Paragraph)
Chromosomal Location
ChrXVI:902044 to 906654 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GDB1 GO evidence and references
  View Computational GO annotations for GDB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 9 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
36 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 1
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 3
  • Positive Genetic: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,536
Molecular Weight (Da) 174,970
Isoelectric Point (pI) 5.58
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:902044 to 906654 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4611 902044..906654 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006388
SUMMARY PARAGRAPH for GDB1

Glycogen is a branched polysaccharide of high molecular mass that is used as a storage carbohydrate. In S. cerevisiae, glycogen is typically catabolized to glucose-1-phosphate and glucose by the Gph1p glycogen phosphorylase and the Gdb1p debranching enzyme (5). However, during sporulation, glycogen is rapidly catabolized to glucose by the Sga1p glucoamylase (5).

The Gdb1p dual-activity glycogen debranching enzyme eliminates branchpoints in glycogen in a two-step process that includes (i) the transfer of a maltotriosyl (or maltosyl) unit from the branchpoint to an adjacent alpha-1,4-glucosyl chain using its oligo-1,4 to 1,4-glucanotransferase activity (EC 2.4.1.25), and (ii) the subsequent hydrolysis of the residual alpha-1,6-linked glucose residue by its alpha-1,6-glucosidase activity (EC 3.2.1.33) (1). Once the branch is resolved, glycogen degradation continues by the action of a second enzyme, the Gph1p glycogen phosphorylase (5).

GDB1 expression is regulated by stress-response elements, and is induced during late exponential growth phase and in response to various stresses, as are other glycogen metabolism genes (1). gdb1 null mutants are viable, but display increased accumulation of glycogen (1). No phenotypes corresponding to the mammalian glycogen storage disease III, associated with mutations in human glycogen debranching enzyme (AGL), have been identified for S. cerevisiae gdb1 null mutants (5).

Last updated: 2005-08-25 Contact SGD

References cited on this page View Complete Literature Guide for GDB1
1) Teste MA, et al.  (2000) The Saccharomyces cerevisiae YPR184w gene encodes the glycogen debranching enzyme. FEMS Microbiol Lett 193(1):105-10
2) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
3) Walkey CJ, et al.  (2011) The Saccharomyces cerevisiae fermentation stress response protein Igd1p/Yfr017p regulates glycogen levels by inhibiting the glycogen debranching enzyme. FEMS Yeast Res 11(6):499-508
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Francois J and Parrou JL  (2001) Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 25(1):125-45