BET2/YPR176C Summary Help

Standard Name BET2 1
Systematic Name YPR176C
Feature Type ORF, Verified
Description Beta subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p (2, 3 and see Summary Paragraph)
Name Description Blocked Early in Transport 1
Chromosomal Location
ChrXVI:892078 to 891101 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BET2 GO evidence and references
  View Computational GO annotations for BET2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
Large-scale survey
reduction of function
59 total interaction(s) for 47 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 4
  • Biochemical Activity: 5
  • Co-purification: 2
  • FRET: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 29
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 6

Expression Summary
Length (a.a.) 325
Molecular Weight (Da) 36,666
Isoelectric Point (pI) 4.89
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:892078 to 891101 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..978 892078..891101 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006380

Bet2p,the beta subunit of the type II (RAB-protein) geranylgeranyl transferase (GGTase) (2, 4) forms the catalytic component (component B) along with Bet4 while Mrs6, the accessory subunit, presents the substrate to these catalytic subunits. GGTase II (Bet2, Bet4, Mrs6) is required for transport between the endoplasmic reticulum and the Golgi, and its role in that pathway seems to be in providing a membrane attachment moiety to Ypt1p and Sec4p, small GTP-binding proteins that help mediate vesicular traffic (1, 2, 4). Bet2p shares sequence similarity with Ram1p, the beta subunit of the yeast farnesyl transferase, and with mammalian Rab geranylgeranyl transferase beta subunits (5, 6).

The type II GGTase is one of three prenyl transferases in yeast, the other two being farnesyl transferase and the type I GGTase (7, 4).

Last updated: 2010-05-18 Contact SGD

References cited on this page View Complete Literature Guide for BET2
1) Newman AP and Ferro-Novick S  (1987) Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection. J Cell Biol 105(4):1587-94
2) Rossi G, et al.  (1991) Dependence of Ypt1 and Sec4 membrane attachment on Bet2. Nature 351(6322):158-61
3) Newman AP, et al.  (1990) BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex. Mol Cell Biol 10(7):3405-14
4) Jiang Y, et al.  (1993) Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p. Nature 366(6450):84-6
5) Armstrong SA, et al.  (1993) cDNA cloning and expression of the alpha and beta subunits of rat Rab geranylgeranyl transferase. J Biol Chem 268(16):12221-9
6) Kohl NE, et al.  (1991) Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases. J Biol Chem 266(28):18884-8
7) Jiang Y, et al.  (1995) Characterization of yeast type-II geranylgeranyltransferase. Methods Enzymol 257:21-9