BET2/YPR176C Summary

Standard Name	BET2 1
Systematic Name	YPR176C
Feature Type	ORF, Verified
Description	Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p (2, 3 and see Summary Paragraph)
Name Description	Blocked Early in Transport 1

Chromosomal Location	ChrXVI:892078 to 891101 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All BET2 GO evidence and references

	View Computational GO annotations for BET2
Molecular Function
Manually curated	Rab geranylgeranyltransferase activity (IDA)
Biological Process
Manually curated	ER to Golgi vesicle-mediated transport (IMP) protein geranylgeranylation (IDA) protein targeting to membrane (IMP)
Cellular Component
Manually curated	Rab-protein geranylgeranyltransferase complex (IDA, IPI)

Mutant phenotypes All BET2 Phenotype evidence and references

Classical genetics
conditional	invertase transport: decreased
Large-scale survey
null	haploinsufficient inviable resistance to clioquinol: decreased
reduction of function	competitive fitness: decreased resistance to hydroxyurea: decreased
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All BET2 Interaction evidence and references

	56 total interaction(s) for 45 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 3 Biochemical Activity: 5 Co-purification: 1 FRET: 2 Reconstituted Complex: 1 Two-hybrid: 2
Genetic Interactions	Negative Genetic: 29 Positive Genetic: 4 Synthetic Growth Defect: 1 Synthetic Lethality: 6
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All BET2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:892078 to 891101 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..978	892078..891101	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000006380

SUMMARY PARAGRAPH for BET2

Bet2p,the beta subunit of the type II (RAB-protein) geranylgeranyl transferase (GGTase) (2, 4) forms the catalytic component (component B) along with Bet4 while Mrs6, the accessory subunit, presents the substrate to these catalytic subunits. GGTase II (Bet2, Bet4, Mrs6) is required for transport between the endoplasmic reticulum and the Golgi, and its role in that pathway seems to be in providing a membrane attachment moiety to Ypt1p and Sec4p, small GTP-binding proteins that help mediate vesicular traffic (1, 2, 4). Bet2p shares sequence similarity with Ram1p, the beta subunit of the yeast farnesyl transferase, and with mammalian Rab geranylgeranyl transferase beta subunits (5, 6).

The type II GGTase is one of three prenyl transferases in yeast, the other two being farnesyl transferase and the type I GGTase (7, 4).

Last updated: 2010-05-18

References cited on this page View Complete Literature Guide for BET2

1)	Newman AP and Ferro-Novick S (1987) Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection. J Cell Biol 105(4):1587-94
2)	Rossi G, et al. (1991) Dependence of Ypt1 and Sec4 membrane attachment on Bet2. Nature 351(6322):158-61
3)	Newman AP, et al. (1990) BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex. Mol Cell Biol 10(7):3405-14
4)	Jiang Y, et al. (1993) Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p. Nature 366(6450):84-6
5)	Armstrong SA, et al. (1993) cDNA cloning and expression of the alpha and beta subunits of rat Rab geranylgeranyl transferase. J Biol Chem 268(16):12221-9
6)	Kohl NE, et al. (1991) Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases. J Biol Chem 266(28):18884-8
7)	Jiang Y, et al. (1995) Characterization of yeast type-II geranylgeranyltransferase. Methods Enzymol 257:21-9