VPS4/YPR173C Summary Help

Standard Name VPS4 1, 2
Systematic Name YPR173C
Alias CSC1 3 , END13 4 , 5 , GRD13 6 , VPL4 7 , VPT10 1 , DID6 8
Feature Type ORF, Verified
Description AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism (9, 10, 11, 12, 13, 14)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXVI:887837 to 886524 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All VPS4 GO evidence and references
  View Computational GO annotations for VPS4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
dominant negative
gain of function
reduction of function
Large-scale survey
267 total interaction(s) for 156 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 6
  • Co-localization: 3
  • Co-purification: 4
  • Far Western: 2
  • PCA: 1
  • Reconstituted Complex: 24
  • Two-hybrid: 28

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 103
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 11
  • Positive Genetic: 9
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 7
  • Synthetic Rescue: 11

Expression Summary
Length (a.a.) 437
Molecular Weight (Da) 48,172
Isoelectric Point (pI) 4.96
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:887837 to 886524 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1314 887837..886524 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006377
References cited on this page View Complete Literature Guide for VPS4
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Shirahama K, et al.  (1997) Mutational analysis of Csc1/Vps4p: involvement of endosome in regulation of autophagy in yeast. Cell Struct Funct 22(5):501-9
4) Munn AL and Riezman H  (1994) Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. J Cell Biol 127(2):373-86
5) Zahn R, et al.  (2001) End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae. J Cell Sci 114(Pt 10):1935-47
6) Nothwehr SF, et al.  (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7
7) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
8) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
9) Babst M, et al.  (1998) The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J 17(11):2982-93
10) Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19
11) Babst M, et al.  (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271-82
12) Wang P, et al.  (2005) AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism. EMBO J 24(17):2989-99
13) Lottridge JM, et al.  (2006) Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body. Proc Natl Acad Sci U S A 103(16):6202-7
14) Azmi I, et al.  (2006) Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. J Cell Biol 172(5):705-17