VPS4/YPR173C Summary Help

VPS4 BASIC INFORMATION

Standard Name VPS4 1, 2
Systematic Name YPR173C
Alias CSC1 3 , END13 4 , 5 , GRD13 6 , VPL4 7 , VPT10 1 , DID6 8
Feature Type ORF, Verified
Description AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism (9, 10, 11, 12, 13, 14)
Name Description Vacuolar Protein Sorting 1, 2
GO Annotations All VPS4 GO evidence and references
    View Computational GO annotations for VPS4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All VPS4 Phenotype details and references
Classical genetics
conditional
dominant negative
gain of function
null
overexpression
reduction of function
Large-scale survey
null
Interactions VPS4 All interactions details and references
111 total interaction(s) for 57 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-Western: 3
  • Co-crystal Structure: 5
  • Co-localization: 3
  • Co-purification: 2
  • Far Western: 2
  • PCA: 1
  • Reconstituted Complex: 19
  • Two-hybrid: 22

Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 7
  • Synthetic Rescue: 5

Sequence Information
ChrXVI:887833 to 886520 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-07-21 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1314 887833..886520 2004-07-21 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000006377

VPS4 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for VPS4]

1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Shirahama K, et al.  (1997) Mutational analysis of Csc1/Vps4p: involvement of endosome in regulation of autophagy in yeast. Cell Struct Funct 22(5):501-9
4) Munn AL and Riezman H  (1994) Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. J Cell Biol 127(2):373-86
5) Zahn R, et al.  (2001) End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae. J Cell Sci 114(Pt 10):1935-47
6) Nothwehr SF, et al.  (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7
7) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
8) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
9) Babst M, et al.  (1998) The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J 17(11):2982-93
10) Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19
11) Babst M, et al.  (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271-82
12) Wang P, et al.  (2005) AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism. EMBO J 24(17):2989-99
13) Lottridge JM, et al.  (2006) Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body. Proc Natl Acad Sci U S A 103(16):6202-7
14) Azmi I, et al.  (2006) Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. J Cell Biol 172(5):705-17