MET16/YPR167C Summary Help

Standard Name MET16 1
Systematic Name YPR167C
Feature Type ORF, Verified
Description 3'-phosphoadenylsulfate reductase; reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism (2 and see Summary Paragraph)
Name Description METhionine requiring 1
Chromosomal Location
ChrXVI:877632 to 876847 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MET16 GO evidence and references
  View Computational GO annotations for MET16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Classical genetics
Large-scale survey
62 total interaction(s) for 49 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 41
  • Positive Genetic: 7
  • Synthetic Growth Defect: 4

Expression Summary
Length (a.a.) 261
Molecular Weight (Da) 30,380
Isoelectric Point (pI) 5.83
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:877632 to 876847 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..786 877632..876847 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006371

MET16 encodes phosphoadenosine-phosphosulfate reductase (3'-phosphoadenylylsulfate reductase; PAPS reductase) (3), an enzyme involved in the sulfate assimilation pathway in yeast (1, 4, 5). MET16 was first identified in a screen for methionine auxotrophs (1), as sulfate assimilation is required for methionine biosynthesis in yeast (4, 3) Transcription of MET16 and other genes required for sulfate assimilation is activated in the absence of methionine. A complex comprising Cbf1p, the transcriptional activator Met4p, and Met28p binds to a UAS in the MET16 promoter (6, 7, 8, 9, 10). Mutations in MET4 abolish transcription of MET16, while mutations in MET1, MET8, and MET17, which encode other sulfate assimilation enzymes, abolish PAPS reductase activity but do not affect transcription of MET16 (3). It has been suggested that the enzymes involved in sulfate assimilation form a complex (3). Met16p is similar to the PAPS reductases from E. coli (3) and Aspergillus nidulans (11).

Last updated: 1999-09-23 Contact SGD

References cited on this page View Complete Literature Guide for MET16
1) Masselot M and De Robichon-Szulmajster H  (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139(2):121-32
2) Schwenn JD, et al.  (1988) Yeast PAPS reductase: properties and requirements of the purified enzyme. Arch Microbiol 150(4):313-9
3) Thomas D, et al.  (1990) Gene-enzyme relationship in the sulfate assimilation pathway of Saccharomyces cerevisiae. Study of the 3'-phosphoadenylylsulfate reductase structural gene. J Biol Chem 265(26):15518-24
4) Masselot M and Surdin-Kerjan Y  (1977) Methionine biosynthesis in Saccharomyces cerevisiae. II. Gene-enzyme relationships in the sulfate assimilation pathway. Mol Gen Genet 154(1):23-30
5) Cooper AJ  (1983) Biochemistry of sulfur-containing amino acids. Annu Rev Biochem 52():187-222
6) Thomas D, et al.  (1992) MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae. Mol Cell Biol 12(4):1719-27
7) Kuras L and Thomas D  (1995) Identification of the yeast methionine biosynthetic genes that require the centromere binding factor 1 for their transcriptional activation. FEBS Lett 367(1):15-8
8) Kuras L and Thomas D  (1995) Functional analysis of Met4, a yeast transcriptional activator responsive to S-adenosylmethionine. Mol Cell Biol 15(1):208-16
9) O'Connell KF, et al.  (1995) Role of the Saccharomyces cerevisiae general regulatory factor CP1 in methionine biosynthetic gene transcription. Mol Cell Biol 15(4):1879-88
10) Kuras L, et al.  (1996) A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism. EMBO J 15(10):2519-29
11) Borges-Walmsley MI, et al.  (1995) Isolation and characterisation of genes for sulphate activation and reduction in Aspergillus nidulans: implications for evolution of an allosteric control region by gene duplication. Mol Gen Genet 247(4):423-9