TIF3/YPR163C Summary Help

Standard Name TIF3
Systematic Name YPR163C
Alias RBL3 , STM1
Feature Type ORF, Verified
Description Translation initiation factor eIF-4B; contains an RNA recognition motif and binds to single-stranded RNA; has RNA annealing activity; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel (1, 2, 3)
Name Description Translation Initiation Factor
Gene Product Alias eIF4B
Chromosomal Location
ChrXVI:869955 to 868645 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TIF3 GO evidence and references
  View Computational GO annotations for TIF3
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
unspecified
Resources
53 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • PCA: 3
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 2
  • Negative Genetic: 4
  • Positive Genetic: 5
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 3
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 436
Molecular Weight (Da) 48,522
Isoelectric Point (pI) 5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:869955 to 868645 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1311 869955..868645 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006367
References cited on this page View Complete Literature Guide for TIF3
1) Coppolecchia R, et al.  (1993) A new yeast translation initiation factor suppresses a mutation in the eIF-4A RNA helicase. EMBO J 12(10):4005-11
2) Altmann M, et al.  (1995) The Saccharomyces cerevisiae translation initiation factor Tif3 and its mammalian homologue, eIF-4B, have RNA annealing activity. EMBO J 14(15):3820-7
3) Walker SE, et al.  (2013) Yeast eIF4B binds to the head of the 40S ribosomal subunit and promotes mRNA recruitment through its N-terminal and internal repeat domains. RNA 19(2):191-207