PIN3/YPR154W Summary Help

Standard Name PIN3 1
Systematic Name YPR154W
Alias LSB2 2
Feature Type ORF, Verified
Description Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; induces the appearance of the [PIN+] prion when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication (1, 3, 4)
Name Description Psi+ INducibility 1
Chromosomal Location
ChrXVI:834565 to 835212 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PIN3 GO evidence and references
  View Computational GO annotations for PIN3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 12 genes
Resources
Large-scale survey
null
overexpression
Resources
75 total interaction(s) for 51 unique genes/features.
Physical Interactions
  • Affinity Capture-Luminescence: 1
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Co-localization: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 50

Genetic Interactions
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 215
Molecular Weight (Da) 23,539
Isoelectric Point (pI) 7.55
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:834565 to 835212 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..648 834565..835212 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006358
References cited on this page View Complete Literature Guide for PIN3
1) Derkatch IL, et al.  (2001) Prions affect the appearance of other prions: the story of [PIN(+)]. Cell 106(2):171-82
2) Madania A, et al.  (1999) The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex. Mol Biol Cell 10(10):3521-38
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Spiess M, et al.  (2013) Lsb1 is a negative regulator of las17 dependent actin polymerization involved in endocytosis. PLoS One 8(4):e61147