PIS1/YPR113W Summary Help

Standard Name PIS1
Systematic Name YPR113W
Feature Type ORF, Verified
Description Phosphatidylinositol synthase; required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins (1, 2)
Name Description Phosphatidyl Inositol Synthase 3
Chromosomal Location
ChrXVI:752257 to 752919 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PIS1 GO evidence and references
  View Computational GO annotations for PIS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 98 genes
Resources
Pathways
Classical genetics
null
repressible
Large-scale survey
null
overexpression
reduction of function
unspecified
Resources
52 total interaction(s) for 50 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • PCA: 23
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 5
  • Positive Genetic: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 220
Molecular Weight (Da) 24,823
Isoelectric Point (pI) 8.92
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:752257 to 752919 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..663 752257..752919 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006317
References cited on this page View Complete Literature Guide for PIS1
1) Nikawa J, et al.  (1987) Primary structure and disruption of the phosphatidylinositol synthase gene of Saccharomyces cerevisiae. J Biol Chem 262(10):4876-81
2) Nikawa J and Yamashita S  (1997) Phosphatidylinositol synthase from yeast. Biochim Biophys Acta 1348(1-2):173-8
3) Nikawa J and Yamashita S  (1984) Molecular cloning of the gene encoding CDPdiacylglycerol-inositol 3-phosphatidyl transferase in Saccharomyces cerevisiae. Eur J Biochem 143(2):251-6