DBF20/YPR111W Summary Help

Standard Name DBF20 1
Systematic Name YPR111W
Feature Type ORF, Verified
Description Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication (2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description DumbBell Forming 7
Chromosomal Location
ChrXVI:747306 to 749000 | ORF Map | GBrowse
Gbrowse
Genetic position: 66 cM
Gene Ontology Annotations All DBF20 GO evidence and references
  View Computational GO annotations for DBF20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
53 total interaction(s) for 40 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 14
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 5
  • Synthetic Growth Defect: 4
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 5
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 564
Molecular Weight (Da) 65,879
Isoelectric Point (pI) 8.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:747306 to 749000 | ORF Map | GBrowse
SGD ORF map
Genetic position: 66 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1999-07-17
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1695 747306..749000 2011-02-03 1999-07-17
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006315
SUMMARY PARAGRAPH for DBF20

DBF20 is a putative serine/threonine-specific protein kinase with a role in cell cycle progression (8). It has 80% identity to DBF2, and was first isolated via low-stringency hybridization of a DBF2 probe to a yeast genomic DNA library (8). Deletion of DBF20 has no growth phenotype by itself, but is lethal in a strain that lacks DBF2 (8). DBF2p and DBF20p carry out at least one essential late-mitotic function. Although a deletion of DBF2 is viable, several alleles of DBF2 are not; the lethality of these alleles can be overcome by overexpression of Spo12p, a factor that interacts with both Dbf2p and Dbf20p (9). Spo12p may therefore be the limiting cofactor that regulates the activities of these two kinases. Dbf20p may also have a role in the regulation of transcription, due to its similarity to Dbf2p which associates with Mob1p and the Ccr4p regulatory complex (10, 11).

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for DBF20
1) Johnston, L.  (1992) Personal Communication, Mortimer Map Edition 11
2) Toyn JH and Johnston LH  (1994) The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition. EMBO J 13(5):1103-13
3) Menssen R, et al.  (2001) Asymmetric spindle pole localization of yeast Cdc15 kinase links mitotic exit and cytokinesis. Curr Biol 11(5):345-50
4) Hwa Lim H, et al.  (2003) Inactivation of mitotic kinase triggers translocation of MEN components to mother-daughter neck in yeast. Mol Biol Cell 14(11):4734-43
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Trcek T, et al.  (2011) Single-molecule mRNA decay measurements reveal promoter- regulated mRNA stability in yeast. Cell 147(7):1484-97
7) Johnston LH and Thomas AP  (1982) The isolation of new DNA synthesis mutants in the yeast Saccharomyces cerevisiae. Mol Gen Genet 186(3):439-44
8) Toyn JH, et al.  (1991) The cell-cycle-regulated budding yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle control. Gene 104(1):63-70
9) Toyn JH and Johnston LH  (1993) Spo12 is a limiting factor that interacts with the cell cycle protein kinases Dbf2 and Dbf20, which are involved in mitotic chromatid disjunction. Genetics 135(4):963-71
10) Komarnitsky SI, et al.  (1998) DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein. Mol Cell Biol 18(4):2100-7
11) Liu HY, et al.  (1997) DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex. EMBO J 16(17):5289-98