PRE2/YPR103W Summary

PRE2 BASIC INFORMATION

Standard Name	PRE2
Systematic Name	YPR103W
Alias	DOA3 , PRG1 , SRR2
Feature Type	ORF, Verified
Description	Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome (1, 2)
Name Description	PRoteinase yscE 3

GO Annotations	All PRE2 GO evidence and references
	View Computational GO annotations for PRE2
Molecular Function
Manually curated	endopeptidase activity (TAS)
Biological Process
Manually curated	ubiquitin-dependent protein catabolic process (IGI)
Cellular Component
Manually curated	proteasome core complex, beta-subunit complex (IPI)

Mutant Phenotype	All PRE2 Phenotype details and references
Classical genetics
conditional	cell cycle progression: abnormal chromosome segregation: abnormal heat sensitivity: increased
null	inviable
reduction of function	UV resistance: decreased inviable mutation frequency: increased ubiquitinated proteins accumulation: increased resistance to canavanine: decreased
Large-scale survey
null	inviable
overexpression	vegetative growth: decreased

Interactions	PRE2 All interactions details and references
	182 total interaction(s) for 62 unique genes/features.
Physical Interactions	Affinity Capture-MS: 67 Affinity Capture-Western: 8 Biochemical Activity: 2 Co-crystal Structure: 3 Co-fractionation: 4 Co-purification: 24 Two-hybrid: 6
Genetic Interactions	Dosage Rescue: 1 Phenotypic Enhancement: 5 Phenotypic Suppression: 3 Synthetic Growth Defect: 15 Synthetic Lethality: 3 Synthetic Rescue: 41

Sequence Information

ChrXVI:732347 to 733210 | |

Last Update

Coordinates: 2004-07-21 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..864	732347..733210	2004-07-21	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000006307

PRE2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for PRE2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PRE2]

1)	Groll M, et al. (1999) The catalytic sites of 20S proteasomes and their role in subunit maturation: a mutational and crystallographic study. Proc Natl Acad Sci U S A 96(20):10976-83
2)	Jager S, et al. (1999) Proteasome beta-type subunits: unequal roles of propeptides in core particle maturation and a hierarchy of active site function. J Mol Biol 291(4):997-1013
3)	Heinemeyer W, et al. (1991) Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival. EMBO J 10(3):555-62

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