SUA7/YPR086W Summary Help

Standard Name SUA7 1
Systematic Name YPR086W
Alias SOH4 2 , 3
Feature Type ORF, Verified
Description Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II (4, 5, 6, 7, 8 and see Summary Paragraph)
Name Description Suppressor of Upstream AUG 1
Gene Product Alias TFIIB 4
Chromosomal Location
ChrXVI:710101 to 711138 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SUA7 GO evidence and references
  View Computational GO annotations for SUA7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 731 genes
Regulators 1 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
reduction of function
Resources
130 total interaction(s) for 76 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 23
  • Biochemical Activity: 1
  • Co-crystal Structure: 5
  • Co-localization: 5
  • Co-purification: 6
  • Far Western: 1
  • PCA: 5
  • Reconstituted Complex: 24
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 11
  • Negative Genetic: 2
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 10
  • Synthetic Rescue: 10

Resources
Expression Summary
histogram
Resources
Length (a.a.) 345
Molecular Weight (Da) 38,200
Isoelectric Point (pI) 8.69
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:710101 to 711138 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1038 710101..711138 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006290
SUMMARY PARAGRAPH for SUA7

SUA7 encodes yeast TFIIB, a general transcription factor required for the initiation of transcription by RNA polymerase II (6, 4). Sua7p is essential for viability (4), and cannot be replaced by human TFIIB (9). The roles of TFIIB and other general transcription factors in basal transcription have been studied extensively in yeast and other eukaryotes, and are reviewed in 10 and 11. Briefly, the TATA binding protein (TBP) binds TATA DNA, and TFIIB binds to the TBP-DNA complex (10, 11). TFIIB binding is required for an RNA polymerase II-TFIIF complex to associate with TBP-DNA; TFIIB forms a bridge between the TBP-DNA complex and the polymerase (10, 11). One cold-sensitive sua7 mutation, E62K, supports complex formation but is severely defective in transcription, suggesting another role for yeast TFIIB in addition to assembling the transcription complex (12). Other non-null sua7 mutations alter transcriptional start site selection (4). A region unique to yeast TFIIB has been identified at the N-terminus of Sua7p; mutations in the yeast-specific region alter transcription in vivo (9).

Last updated: 2000-01-04 Contact SGD

References cited on this page View Complete Literature Guide for SUA7
1) Hampsey M, et al.  (1991) Extragenic suppressors of a translation initiation defect in the cyc1 gene of Saccharomyces cerevisiae. Biochimie 73(12):1445-55
2) Fan HY and Klein HL  (1994) Characterization of mutations that suppress the temperature-sensitive growth of the hpr1 delta mutant of Saccharomyces cerevisiae. Genetics 137(4):945-56
3) Fan HY, et al.  (1996) Mutations in the RNA polymerase II transcription machinery suppress the hyperrecombination mutant hpr1 delta of Saccharomyces cerevisiae. Genetics 142(3):749-59
4) Pinto I, et al.  (1992) The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo. Cell 68(5):977-88
5) Pinto I, et al.  (1994) Characterization of sua7 mutations defines a domain of TFIIB involved in transcription start site selection in yeast. J Biol Chem 269(48):30569-73
6) Tschochner H, et al.  (1992) Yeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human transcription factor IIB. Proc Natl Acad Sci U S A 89(23):11292-6
7) Wu WH, et al.  (1999) Mutational analysis of yeast TFIIB. A functional relationship between Ssu72 and Sub1/Tsp1 defined by allele-specific interactions with TFIIB. Genetics 153(2):643-52
8) Chen BS and Hampsey M  (2004) Functional interaction between TFIIB and the Rpb2 subunit of RNA polymerase II: implications for the mechanism of transcription initiation. Mol Cell Biol 24(9):3983-91
9) Shaw SP, et al.  (1996) Identifying a species-specific region of yeast TF11B in vivo. Mol Cell Biol 16(7):3651-7
10) Reinberg D, et al.  (1998) The RNA polymerase II general transcription factors: past, present, and future. Cold Spring Harb Symp Quant Biol 63:83-103
11) Kornberg RD  (1998) Mechanism and regulation of yeast RNA polymerase II transcription. Cold Spring Harb Symp Quant Biol 63:229-32
12) Cho EJ and Buratowski S  (1999) Evidence that transcription factor IIB is required for a post-assembly step in transcription initiation. J Biol Chem 274(36):25807-13