OPY2/YPR075C Summary Help

Standard Name OPY2 1
Systematic Name YPR075C
Feature Type ORF, Verified
Description Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress (1, 2, 3, 4)
Name Description Overproduction-induced Pheromone-resistant Yeast 1
Chromosomal Location
ChrXVI:696819 to 695737 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All OPY2 GO evidence and references
  View Computational GO annotations for OPY2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
74 total interaction(s) for 59 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Co-purification: 1
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 7

Genetic Interactions
  • Negative Genetic: 39
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 360
Molecular Weight (Da) 38,901
Isoelectric Point (pI) 4.23
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:696819 to 695737 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1083 696819..695737 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006279
References cited on this page View Complete Literature Guide for OPY2
1) Edwards MC, et al.  (1997) Human CPR (cell cycle progression restoration) genes impart a Far- phenotype on yeast cells. Genetics 147(3):1063-76
2) Wu C, et al.  (2006) Adaptor protein Ste50p links the Ste11p MEKK to the HOG pathway through plasma membrane association. Genes Dev 20(6):734-46
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Karunanithi S and Cullen PJ  (2012) The filamentous growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 transcriptional repressors in Saccharomyces cerevisiae. Genetics 192(3):869-87