NOT5/YPR072W Summary Help

Standard Name NOT5
Systematic Name YPR072W
Feature Type ORF, Verified
Description Subunit of CCR4-NOT global transcriptional regulator; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not5p and Not3p is mutated in cancers (1, 2, 3)
Chromosomal Location
ChrXVI:690107 to 691789 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NOT5 GO evidence and references
  View Computational GO annotations for NOT5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
133 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 39
  • Biochemical Activity: 4
  • Co-fractionation: 2
  • Co-purification: 1
  • PCA: 6
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 7
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 18
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 560
Molecular Weight (Da) 65,854
Isoelectric Point (pI) 5.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:690107 to 691789 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1683 690107..691789 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006276
References cited on this page View Complete Literature Guide for NOT5
1) Oberholzer U and Collart MA  (1998) Characterization of NOT5 that encodes a new component of the Not protein complex. Gene 207(1):61-9
2) Chen J, et al.  (2001) Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J Mol Biol 314(4):683-94
3) Collart MA, et al.  (2013) The Not3/5 subunit of the Ccr4-Not complex: a central regulator of gene expression that integrates signals between the cytoplasm and the nucleus in eukaryotic cells. Cell Signal 25(4):743-51