Standard Name	NOT5
Systematic Name	YPR072W
Feature Type	ORF, Verified
Description	Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation (1, 2)

Chromosomal Location	ChrXVI:690107 to 691789 | ORF Map | GBrowse

	View Computational GO annotations for NOT5
Molecular Function
Manually curated	contributes_to ubiquitin-protein ligase activity (IMP)
Biological Process
Manually curated	deadenylation-independent decapping of nuclear-transcribed mRNA (IMP) nuclear-transcribed mRNA poly(A) tail shortening (IDA) positive regulation of transcription elongation from RNA polymerase II promoter (IDA, IPI) protein ubiquitination (IMP) regulation of transcription from RNA polymerase II promoter (IPI) transcription elongation from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	CCR4-NOT core complex (IPI) cytoplasm (IDA)

Classical genetics
null	RNR4 mRNA accumulation: decreased RNR2 mRNA accumulation: decreased RNR3 mRNA accumulation: decreased resistance to hydroxyurea: decreased resistance to zymocin: decreased viable
Large-scale survey
null	budding pattern: abnormal cell shape: abnormal glycogen accumulation: increased polyphosphate accumulation: abnormal competitive fitness: decreased filamentous growth: increased heat sensitivity: increased invasive growth: absent resistance to ethanol: decreased resistance to 4-chlorophenol: decreased resistance to sulfanilamide: decreased resistance to L-1,4-dithiothreitol: decreased resistance to doxorubicin: decreased resistance to hygromycin B: decreased resistance to sorbate: decreased resistance to St. John's Wort extract: decreased toxin resistance: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	128 total interaction(s) for 62 unique genes/features.
Physical Interactions	Affinity Capture-MS: 17 Affinity Capture-RNA: 2 Affinity Capture-Western: 38 Biochemical Activity: 4 Co-fractionation: 2 Co-purification: 1 PCA: 6 Protein-peptide: 1 Reconstituted Complex: 1 Two-hybrid: 16
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 5 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Positive Genetic: 1 Synthetic Growth Defect: 7 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 18 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:690107 to 691789 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1683 690107..691789 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006276