NOT5/YPR072W Summary

NOT5 BASIC INFORMATION

Standard Name	NOT5
Systematic Name	YPR072W
Feature Type	ORF, Verified
Description	Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation (1, 2)

GO Annotations	All NOT5 GO evidence and references
	View Computational GO annotations for NOT5
Molecular Function
Manually curated	contributes_to ubiquitin-protein ligase activity (IMP)
Biological Process
Manually curated	nuclear-transcribed mRNA poly(A) tail shortening (IDA) protein ubiquitination (IMP) regulation of transcription from RNA polymerase II promoter (IPI) RNA elongation from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	CCR4-NOT core complex (IPI) cytoplasm (IDA)

Mutant Phenotype	All NOT5 Phenotype details and references
Classical genetics
null	RNR2 mRNA accumulation: decreased RNR3 mRNA accumulation: decreased RNR4 mRNA accumulation: decreased resistance to hydroxyurea: decreased resistance to zymocin: decreased viable
Large-scale survey
null	budding pattern: abnormal cell shape: abnormal glycogen accumulation: increased polyphosphate accumulation: abnormal competitive fitness: decreased filamentous growth: increased invasive growth: absent resistance to L-1,4-dithiothreitol: decreased resistance to sulfanilamide: decreased resistance to St. John's Wort extract: decreased resistance to doxorubicin: decreased resistance to ethanol: decreased toxin resistance: decreased viable

Interactions	NOT5 All interactions details and references
	100 total interaction(s) for 48 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-RNA: 1 Affinity Capture-Western: 31 Biochemical Activity: 4 Co-fractionation: 1 Co-purification: 1 PCA: 5 Protein-peptide: 1 Reconstituted Complex: 1 Two-hybrid: 9
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 4 Phenotypic Enhancement: 2 Synthetic Growth Defect: 7 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 17 Synthetic Rescue: 3

Sequence Information

ChrXVI:690104 to 691786 | |

Last Update

Coordinates: 2004-07-21 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1683	690104..691786	2004-07-21	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000006276

NOT5 RESOURCES

Click on map for expanded view

SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for NOT5

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NOT5]

1)	Oberholzer U and Collart MA (1998) Characterization of NOT5 that encodes a new component of the Not protein complex. Gene 207(1):61-9
2)	Chen J, et al. (2001) Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J Mol Biol 314(4):683-94

SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.