Standard Name	MED1
Systematic Name	YPR070W
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description	MEDiator complex

Chromosomal Location	ChrXVI:685898 to 687598 | ORF Map | GBrowse

	View Computational GO annotations for MED1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	core mediator complex (IDA)

Classical genetics
null	subcellular morphology: abnormal transposable element transposition: increased
Large-scale survey
null	Protein A-nonstop reporter mRNA accumulation: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased haploinsufficient heat sensitivity: increased metal resistance: decreased Rad52-YFP distribution: increased resistance to methyl methanesulfonate: decreased resistance to nickel(2+): decreased resistance to sirolimus: increased resistance to camptothecin: increased resistance to cycloheximide: decreased resistance to dihydromotuporamine C: decreased resistance to neothyonidioside: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to hydroxyurea: decreased resistance to tunicamycin: decreased resistance to formaldehyde: decreased stress resistance: increased telomere length: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	590 total interaction(s) for 301 unique genes/features.
Physical Interactions	Affinity Capture-MS: 88 Affinity Capture-Western: 29 Co-purification: 6 Reconstituted Complex: 7 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 376 Phenotypic Enhancement: 1 Phenotypic Suppression: 4 Positive Genetic: 32 Synthetic Growth Defect: 30 Synthetic Lethality: 6 Synthetic Rescue: 7
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:685898 to 687598 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1701 685898..687598 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006274