MED1/YPR070W Summary Help

Standard Name MED1
Systematic Name YPR070W
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description MEDiator complex
Chromosomal Location
ChrXVI:685898 to 687598 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MED1 GO evidence and references
  View Computational GO annotations for MED1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
null
Large-scale survey
null
overexpression
Resources
593 total interaction(s) for 301 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 88
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 29
  • Co-purification: 7
  • Reconstituted Complex: 7
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 376
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 4
  • Positive Genetic: 33
  • Synthetic Growth Defect: 30
  • Synthetic Lethality: 6
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 566
Molecular Weight (Da) 64,251
Isoelectric Point (pI) 4.52
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:685898 to 687598 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1701 685898..687598 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006274
References cited on this page View Complete Literature Guide for MED1
1) Balciunas D, et al.  (1999) The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression. Proc Natl Acad Sci U S A 96(2):376-81
2) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9