ROX1/YPR065W Summary Help

Standard Name ROX1 1
Systematic Name YPR065W
Alias REO1 2 , 3
Feature Type ORF, Verified
Description Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance (4, 5, 6, 7, 8, 9)
Name Description Regulation by OXygen 1
Chromosomal Location
ChrXVI:679693 to 680799 | ORF Map | GBrowse
Gene Ontology Annotations All ROX1 GO evidence and references
  View Computational GO annotations for ROX1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 71 genes
Regulators 9 genes
Classical genetics
Large-scale survey
85 total interaction(s) for 78 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • PCA: 1

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 58
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 4
  • Positive Genetic: 8
  • Synthetic Growth Defect: 1

Expression Summary
Length (a.a.) 368
Molecular Weight (Da) 41,838
Isoelectric Point (pI) 10.72
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:679693 to 680799 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1107 679693..680799 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006269
References cited on this page View Complete Literature Guide for ROX1
1) Lowry CV and Zitomer RS  (1984) Oxygen regulation of anaerobic and aerobic genes mediated by a common factor in yeast. Proc Natl Acad Sci U S A 81(19):6129-33
2) Kwast KE, et al.  (1997) REO1 and ROX1 are alleles of the same gene which encodes a transcriptional repressor of hypoxic genes in Saccharomyces cerevisiae. Curr Genet 32(6):377-83
3) Trueblood CE and Poyton RO  (1988) Identification of REO1, a gene involved in negative regulation of COX5b and ANB1 in aerobically grown Saccharomyces cerevisiae. Genetics 120(3):671-80
4) Deckert J, et al.  (1995) Multiple elements and auto-repression regulate Rox1, a repressor of hypoxic genes in Saccharomyces cerevisiae. Genetics 139(3):1149-58
5) Keng T  (1992) HAP1 and ROX1 form a regulatory pathway in the repression of HEM13 transcription in Saccharomyces cerevisiae. Mol Cell Biol 12(6):2616-23
6) Deckert J, et al.  (1995) Mutational analysis of Rox1, a DNA-bending repressor of hypoxic genes in Saccharomyces cerevisiae. Mol Cell Biol 15(11):6109-17
7) Ter Linde JJ and Steensma HY  (2002) A microarray-assisted screen for potential Hap1 and Rox1 target genes in Saccharomyces cerevisiae. Yeast 19(10):825-40
8) Montanes FM, et al.  (2011) Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors. Mol Microbiol 79(4):1008-23
9) Liu J and Barrientos A  (2013) Transcriptional regulation of yeast oxidative phosphorylation hypoxic genes by oxidative stress. Antioxid Redox Signal 19(16):1916-27
10) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
11) Balasubramanian B, et al.  (1993) The Rox1 repressor of the Saccharomyces cerevisiae hypoxic genes is a specific DNA-binding protein with a high-mobility-group motif. Mol Cell Biol 13(10):6071-8