SMK1/YPR054W Summary Help

Standard Name SMK1 1
Systematic Name YPR054W
Feature Type ORF, Verified
Description Middle sporulation-specific mitogen-activated protein kinase (MAPK); required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p (1, 2, 3, 4)
Chromosomal Location
ChrXVI:666280 to 667446 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SMK1 GO evidence and references
  View Computational GO annotations for SMK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 9 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
50 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 1
  • Negative Genetic: 7
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 8
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 388
Molecular Weight (Da) 44,300
Isoelectric Point (pI) 8.87
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:666280 to 667446 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1167 666280..667446 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006258
References cited on this page View Complete Literature Guide for SMK1
1) Krisak L, et al.  (1994) SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae. Genes Dev 8(18):2151-61
2) Wagner M, et al.  (1999) Distinct steps in yeast spore morphogenesis require distinct SMK1 MAP kinase thresholds. Genetics 151(4):1327-40
3) Pierce M, et al.  (1998) Transcriptional regulation of the SMK1 mitogen-activated protein kinase gene during meiotic development in Saccharomyces cerevisiae. Mol Cell Biol 18(10):5970-80
4) Huang LS, et al.  (2005) The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 102(35):12431-6