PUF2/YPR042C Summary Help

Standard Name PUF2 1
Systematic Name YPR042C
Feature Type ORF, Verified
Description PUF family mRNA-binding protein; Pumilio homology domain confers RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; binding site composed of two UAAU tetranucleotides, separated by a 3-nt linker; PUF2 has a paralog, JSN1, that arose from the whole genome duplication (2, 3, 4)
Name Description PUmilio-homology domain Family 1
Chromosomal Location
ChrXVI:653662 to 650435 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PUF2 GO evidence and references
  View Computational GO annotations for PUF2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Large-scale survey
null
Resources
40 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • PCA: 1
  • Protein-RNA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 9
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 6
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,075
Molecular Weight (Da) 119,507
Isoelectric Point (pI) 9.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:653662 to 650435 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3228 653662..650435 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006246
References cited on this page View Complete Literature Guide for PUF2
1) Olivas W and Parker R  (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
2) Gerber AP, et al.  (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Yosefzon Y, et al.  (2011) Divergent RNA binding specificity of yeast Puf2p. RNA 17(8):1479-88