TIF5/YPR041W Summary Help

Standard Name TIF5
Systematic Name YPR041W
Alias SUI5 1
Feature Type ORF, Verified
Description Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 (2, 3, 4)
Name Description Translation Initiation Factor
Chromosomal Location
ChrXVI:648704 to 649921 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TIF5 GO evidence and references
  View Computational GO annotations for TIF5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Resources
Classical genetics
conditional
reduction of function
Large-scale survey
null
overexpression
reduction of function
Resources
229 total interaction(s) for 90 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 85
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 43
  • Co-fractionation: 7
  • Co-purification: 3
  • Far Western: 1
  • PCA: 2
  • Reconstituted Complex: 30
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 4
  • Negative Genetic: 28
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Positive Genetic: 2
  • Synthetic Lethality: 5
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 405
Molecular Weight (Da) 45,261
Isoelectric Point (pI) 4.78
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:648704 to 649921 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1218 648704..649921 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006245
References cited on this page View Complete Literature Guide for TIF5
1) Huang HK, et al.  (1997) GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae. Genes Dev 11(18):2396-413
2) Das S, et al.  (2001) Eukaryotic translation initiation factor 5 functions as a GTPase-activating protein. J Biol Chem 276(9):6720-6
3) Asano K, et al.  (2000) A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo. Genes Dev 14(19):2534-46
4) Jennings MD and Pavitt GD  (2010) eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation. Nature 465(7296):378-81