Standard Name	APL4
Systematic Name	YPR029C
Feature Type	ORF, Verified
Description	Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport (1, 2)
Name Description	clathrin Adaptor Protein complex Large chain

Chromosomal Location	ChrXVI:626967 to 624469 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for APL4
Molecular Function
Manually curated	clathrin binding (IPI, ISS)
Biological Process
Manually curated	Golgi to vacuole transport (IMP) vesicle-mediated transport (IPI, ISS)
Cellular Component
Manually curated	AP-1 adaptor complex (IPI, ISS)

Classical genetics
null	vacuolar morphology: abnormal vacuolar transport: abnormal
reduction of function	vacuolar transport: decreased
Large-scale survey
null	competitive fitness: decreased endocytosis: decreased haploinsufficient resistance to ciclopirox olamine: decreased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	146 total interaction(s) for 95 unique genes/features.
Physical Interactions	Affinity Capture-MS: 37 Affinity Capture-Western: 6 Biochemical Activity: 1 Co-purification: 1 PCA: 10 Reconstituted Complex: 2 Two-hybrid: 6
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 58 Phenotypic Enhancement: 1 Positive Genetic: 15 Synthetic Growth Defect: 4 Synthetic Lethality: 2 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:626967 to 624469 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2499 626967..624469 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006233