RPA135/YPR010C Summary

RPA135 BASIC INFORMATION

Standard Name	RPA135 1
Systematic Name	YPR010C
Alias	RPA2 , RRN2 2 , SRP3 1 , 3
Feature Type	ORF, Verified
Description	RNA polymerase I subunit A135 (1, 2)
Name Description	RNA Polymerase A 1
Gene Product Alias	A135 4

GO Annotations	All RPA135 GO evidence and references
	View Computational GO annotations for RPA135
Molecular Function
Manually curated	contributes_to DNA-directed RNA polymerase activity (IDA)
Biological Process
Manually curated	transcription of nuclear rRNA large RNA polymerase I transcript (IGI)
Cellular Component
Manually curated	DNA-directed RNA polymerase I complex (IDA)

Mutant Phenotype	All RPA135 Phenotype details and references
Classical genetics
conditional	heat sensitivity: increased
null	rRNAs derived from 37S primary transcript accumulation: decreased inviable
reduction of function	rRNA processing intermediates accumulation: abnormal 60S large ribosomal subunit accumulation: decreased
Large-scale survey
null	inviable

Interactions	RPA135 All interactions details and references
	137 total interaction(s) for 68 unique genes/features.
Physical Interactions	Affinity Capture-MS: 106 Affinity Capture-Western: 9 Biochemical Activity: 1 Co-purification: 2 PCA: 2 Reconstituted Complex: 1 Two-hybrid: 9
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Phenotypic Enhancement: 1 Synthetic Lethality: 2 Synthetic Rescue: 2

Sequence Information

ChrXVI:581193 to 577582 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-07-21 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3612	581193..577582	2004-07-21	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000006214

RPA135 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for RPA135

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
RFA2	Nomenclature conflict	RPA2 has been used to refer to both RFA2/YNL312W, a subunit of the replication factor A, and RPA135/YPR010C, a subunit of RNA polymerase I.

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPA135]

1)	Yano R and Nomura M (1991) Suppressor analysis of temperature-sensitive mutations of the largest subunit of RNA polymerase I in Saccharomyces cerevisiae: a suppressor gene encodes the second-largest subunit of RNA polymerase I. Mol Cell Biol 11(2):754-64
2)	Nogi Y, et al. (1991) An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88(16):7026-30
3)	McCusker JH, et al. (1991) Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes. Mol Cell Biol 11(2):746-53
4)	Riva M, et al. (1982) Natural variation in yeast RNA polymerase A. Formation of a mosaic RNA polymerase A in a meiotic segregant from an interspecific hybrid. J Biol Chem 257(8):4570-7

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