KAR9/YPL269W Summary Help

Standard Name KAR9 1
Systematic Name YPL269W
Feature Type ORF, Verified
Description Karyogamy protein; required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules; localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase (1, 2)
Name Description KARyogamy 1
Chromosomal Location
ChrXVI:33013 to 34947 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All KAR9 GO evidence and references
  View Computational GO annotations for KAR9
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
246 total interaction(s) for 108 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 15
  • Biochemical Activity: 2
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 28

Genetic Interactions
  • Negative Genetic: 61
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 61
  • Synthetic Lethality: 49
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 644
Molecular Weight (Da) 74,244
Isoelectric Point (pI) 9.16
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:33013 to 34947 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1935 33013..34947 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006190
References cited on this page View Complete Literature Guide for KAR9
1) Miller RK and Rose MD  (1998) Kar9p is a novel cortical protein required for cytoplasmic microtubule orientation in yeast. J Cell Biol 140(2):377-90
2) Miller RK, et al.  (1999) The cortical localization of the microtubule orientation protein, Kar9p, is dependent upon actin and proteins required for polarization. J Cell Biol 144(5):963-75