GYP5/YPL249C Summary Help

Standard Name GYP5 1
Systematic Name YPL249C
Feature Type ORF, Verified
Description GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; GYP5 has a paralog, GYL1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Gtpase-activating protein for Ypt Proteins 1
Chromosomal Location
ChrXVI:79353 to 76669 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All GYP5 GO evidence and references
  View Computational GO annotations for GYP5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Large-scale survey
null
Resources
119 total interaction(s) for 73 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-Western: 8
  • Biochemical Activity: 1
  • Co-fractionation: 3
  • Co-localization: 2
  • Far Western: 3
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 68

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 7
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 894
Molecular Weight (Da) 101,799
Isoelectric Point (pI) 4.55
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:79353 to 76669 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2685 79353..76669 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006170
References cited on this page View Complete Literature Guide for GYP5
1) De Antoni A, et al.  (2002) Significance of GTP hydrolysis in Ypt1p-regulated endoplasmic reticulum to Golgi transport revealed by the analysis of two novel Ypt1-GAPs. J Biol Chem 277(43):41023-31
2) Bon E, et al.  (2000) A network of proteins around Rvs167p and Rvs161p, two proteins related to the yeast actin cytoskeleton. Yeast 16(13):1229-41
3) Friesen H, et al.  (2005) Interaction of the Saccharomyces cerevisiae cortical actin patch protein Rvs167p with proteins involved in ER to Golgi vesicle trafficking. Genetics 170(2):555-68
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Prigent M, et al.  (2011) The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis. Traffic 12(8):1084-97
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76