RVB2/YPL235W Summary Help

Standard Name RVB2 1
Systematic Name YPL235W
Alias TIP49B 2 , TIH2 3 , TIP48 4
Feature Type ORF, Verified
Description ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly (1, 2, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
Name Description RuVB-like 5
Chromosomal Location
ChrXVI:103232 to 104647 | ORF Map | GBrowse
Gene Ontology Annotations All RVB2 GO evidence and references
  View Computational GO annotations for RVB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
320 total interaction(s) for 194 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 183
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 17
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 4
  • Reconstituted Complex: 12
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Negative Genetic: 49
  • Positive Genetic: 14
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 471
Molecular Weight (Da) 51,611
Isoelectric Point (pI) 5.06
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:103232 to 104647 | ORF Map | GBrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1416 103232..104647 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006156
References cited on this page View Complete Literature Guide for RVB2
1) Shen X, et al.  (2000) A chromatin remodelling complex involved in transcription and DNA processing. Nature 406(6795):541-4
2) Kanemaki M, et al.  (1999) TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a. J Biol Chem 274(32):22437-44
3) Lim CR, et al.  (2000) The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription. J Biol Chem 275(29):22409-17
4) Wood MA, et al.  (2000) An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. Mol Cell 5(2):321-30
5) Jonsson ZO, et al.  (2001) Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes. J Biol Chem 276(19):16279-88
6) King TH, et al.  (2001) A well-connected and conserved nucleoplasmic helicase is required for production of box C/D and H/ACA snoRNAs and localization of snoRNP proteins. Mol Cell Biol 21(22):7731-46
7) Krogan NJ, et al.  (2003) A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1. Mol Cell 12(6):1565-76
8) Mizuguchi G, et al.  (2004) ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex. Science 303(5656):343-8
9) Jonsson ZO, et al.  (2004) Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex. Mol Cell 16(3):465-77
10) Zhao R, et al.  (2005) Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone. Cell 120(5):715-27
11) Gribun A, et al.  (2008) Yeast Rvb1 and Rvb2 are ATP-dependent DNA helicases that form a heterohexameric complex. J Mol Biol 376(5):1320-33
12) Torreira E, et al.  (2008) Architecture of the pontin/reptin complex, essential in the assembly of several macromolecular complexes. Structure 16(10):1511-20
13) Zhao R, et al.  (2008) Molecular chaperone Hsp90 stabilizes Pih1/Nop17 to maintain R2TP complex activity that regulates snoRNA accumulation. J Cell Biol 180(3):563-78
14) Kakihara Y and Houry WA  (2012) The R2TP complex: discovery and functions. Biochim Biophys Acta 1823(1):101-7