TYW1/YPL207W Summary Help

Standard Name TYW1 1
Systematic Name YPL207W
Feature Type ORF, Verified
Description Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron (2, 3)
Name Description Trna-YW synthesizing protein 1
Chromosomal Location
ChrXVI:159909 to 162341 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TYW1 GO evidence and references
  View Computational GO annotations for TYW1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
overexpression
Resources
60 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Co-purification: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 32
  • Positive Genetic: 2
  • Synthetic Growth Defect: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 810
Molecular Weight (Da) 89,805
Isoelectric Point (pI) 8.11
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:159909 to 162341 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2433 159909..162341 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006128
References cited on this page View Complete Literature Guide for TYW1
1) Noma A, et al.  (2006) Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J 25(10):2142-54
2) Noma A and Suzuki T  (2006) Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp Ser (Oxf) (50):65-6
3) Li L, et al.  (2011) Yap5 protein-regulated transcription of the TYW1 gene protects yeast from high iron toxicity. J Biol Chem 286(44):38488-97