HRR25/YPL204W Summary Help

Standard Name HRR25 1
Systematic Name YPL204W
Alias KTI14 2 , 3
Feature Type ORF, Verified
Description Protein kinase; involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; phosphorylates the COPII coat; phosphorylates Tif6p which plays a critical role in 60S ribosomal subunit biogenesis; interacts with Sit4p phosphatase; homolog of mammalian casein kinase 1delta (CK1delta) (4, 5, 6, 7, 8)
Name Description HO and Radiation Repair 1
Chromosomal Location
ChrXVI:164276 to 165760 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All HRR25 GO evidence and references
  View Computational GO annotations for HRR25
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
conditional
null
reduction of function
repressible
unspecified
Large-scale survey
conditional
null
overexpression
repressible
Resources
301 total interaction(s) for 220 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 173
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 18
  • Biochemical Activity: 26
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 41
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 22
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 494
Molecular Weight (Da) 57,340
Isoelectric Point (pI) 10.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:164276 to 165760 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1485 164276..165760 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006125
References cited on this page View Complete Literature Guide for HRR25
1) Hoekstra MF, et al.  (1991) HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA. Science 253(5023):1031-4
2) Butler AR, et al.  (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14(9):6306-16
3) Mehlgarten C and Schaffrath R  (2003) Mutant casein kinase I (Hrr25p/Kti14p) abrogates the G1 cell cycle arrest induced by Kluyveromyces lactiszymocin in budding yeast. Mol Genet Genomics 269(2):188-96
4) Milne DM, et al.  (2001) Catalytic activity of protein kinase CK1 delta (casein kinase 1delta) is essential for its normal subcellular localization. Exp Cell Res 263(1):43-54
5) Phatnani HP, et al.  (2004) Expanding the functional repertoire of CTD kinase I and RNA polymerase II: novel phosphoCTD-associating proteins in the yeast proteome. Biochemistry 43(50):15702-19
6) Ray P, et al.  (2008) The Saccharomyces cerevisiae 60 S Ribosome Biogenesis Factor Tif6p Is Regulated by Hrr25p-mediated Phosphorylation. J Biol Chem 283(15):9681-91
7) Lord C, et al.  (2011) Sequential interactions with Sec23 control the direction of vesicle traffic. Nature 473(7346):181-6
8) Bhandari D, et al.  (2013) Sit4p/PP6 regulates ER-to-Golgi traffic by controlling the dephosphorylation of COPII coat subunits. Mol Biol Cell 24(17):2727-38