TCO89/YPL180W Summary Help

Standard Name TCO89 1
Systematic Name YPL180W
Feature Type ORF, Verified
Description Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin (1, 2 and see Summary Paragraph)
Name Description Tor Complex One 1
Chromosomal Location
ChrXVI:205248 to 207647 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TCO89 GO evidence and references
  View Computational GO annotations for TCO89
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
234 total interaction(s) for 176 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 29
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 10
  • Co-purification: 1
  • PCA: 6
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 141
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 15
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 4
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 799
Molecular Weight (Da) 88,844
Isoelectric Point (pI) 8.29
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:205248 to 207647 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2400 205248..207647 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006101
SUMMARY PARAGRAPH for TCO89

TCO89 encodes a nonessential protein that is a component of the TOR complex 1 (TORC1; 1). TORC1 mediates cell growth in response to nutrient availability and cellular stresses by regulating protein synthesis, ribosome biogenesis, autophagy, meiosis, cell cycling, nutrient permease sorting and turnover, and transcriptional activation (3, 4, 5, 6, 7, 8, 3, 9, 10, 11). However, for transcriptional regulation, the presence of Tco89p in TORC1 appears to be dispensable (1). In addition to Tco89p, TORC1 consists of Lst8p, Kog1p, and either Tor1p or Tor2p (4, 1). Mutations in TCO89 are synthetically lethal in combination with tor1 mutations and loss of Tco89p leads to rapamycin hypersensitivity and defects in cell wall integrity (1). Similarly to Tor1p, Tor2p, and Lst8p, Tco89p localizes to the inner side of the plasma membrane, but unlike the other TORC1 components Tco89p can also be found surrounding the vacuolar membrane. This distinct pattern of localization suggests that Tco89p may have an additional function separate from its role as a component of TORC1 (1).

Last updated: 2005-11-03 Contact SGD

References cited on this page View Complete Literature Guide for TCO89
1) Reinke A, et al.  (2004) TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem 279(15):14752-62
2) Chen H, et al.  (2012) The histone H3 lysine 56 acetylation pathway is regulated by target of rapamycin (TOR) signaling and functions directly in ribosomal RNA biogenesis. Nucleic Acids Res 40(14):6534-46
3) Cardenas ME, et al.  (1999) The TOR signaling cascade regulates gene expression in response to nutrients. Genes Dev 13(24):3271-9
4) Loewith R, et al.  (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10(3):457-68
5) Weisman R and Choder M  (2001) The fission yeast TOR homolog, tor1+, is required for the response to starvation and other stresses via a conserved serine. J Biol Chem 276(10):7027-32
6) Barbet NC, et al.  (1996) TOR controls translation initiation and early G1 progression in yeast. Mol Biol Cell 7(1):25-42
7) Powers T and Walter P  (1999) Regulation of ribosome biogenesis by the rapamycin-sensitive TOR-signaling pathway in Saccharomyces cerevisiae. Mol Biol Cell 10(4):987-1000
8) Kamada Y, et al.  (2000) Tor-mediated induction of autophagy via an Apg1 protein kinase complex. J Cell Biol 150(6):1507-13
9) Zheng XF and Schreiber SL  (1997) Target of rapamycin proteins and their kinase activities are required for meiosis. Proc Natl Acad Sci U S A 94(7):3070-5
10) Heitman J, et al.  (1991) Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast. Science 253(5022):905-9
11) Schmidt A, et al.  (1998) The TOR nutrient signalling pathway phosphorylates NPR1 and inhibits turnover of the tryptophan permease. EMBO J 17(23):6924-31