REV3/YPL167C Summary Help

Standard Name REV3 1
Systematic Name YPL167C
Alias PSO1 2 , 3
Feature Type ORF, Verified
Description Catalytic subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev7p, Pol31p and Pol32p (4, 5, 6, 7 and see Summary Paragraph)
Name Description REVersionless 1
Chromosomal Location
ChrXVI:237107 to 232593 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -102 cM
Gene Ontology Annotations All REV3 GO evidence and references
  View Computational GO annotations for REV3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Resources
246 total interaction(s) for 91 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-Western: 10
  • Co-purification: 2
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 37
  • Phenotypic Enhancement: 53
  • Phenotypic Suppression: 53
  • Positive Genetic: 1
  • Synthetic Growth Defect: 48
  • Synthetic Lethality: 12
  • Synthetic Rescue: 12

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,504
Molecular Weight (Da) 172,956
Isoelectric Point (pI) 8.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:237107 to 232593 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -102 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4515 237107..232593 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006088
SUMMARY PARAGRAPH for REV3

REV3 and REV7 are involved in translesion synthesis during post-replication repair and double-strand break repair (4, 8). REV3 and REV7 encode the subunits of DNA polymerase zeta; Rev3p is the catalytic subunit whose activity is stimulated by Rev7p (9). In addition to their role in translesion synthesis, Rev3p and Rev7p are involved in double-strand break repair (10).

Pol zeta is an inefficient DNA polymerase; it has low fidelity and processivity when incorporating nucleotides across from damaged and undamaged base pairs (9, 11, 12, 13, 4). However, it cooperates with different DNA polymerases, such as Rev1p, pol eta (encoded by RAD30), and the catalytic subunit of pol delta (encoded by POL3), to allow efficient DNA synthesis from the termini of mispaired nucleotides (14, 15, 16, 4). This process can be error-free or error-prone depending on the damaged nucleotide and the inserted nucleotide (reviewed in 4). However, REV3 and REV7 are primarily considered to be in an error-prone translesion pathway due to a decrease in mutation frequency in their absence (1, 17, 18).

REV3 and REV7 are also implicated in double-strand break repair (5). A rev3 mutant has lower levels of mutations associated with homologous recombination events (19). The Rev1p-polzeta complex localizes to double-strand break sites (10).

Pol zeta is widely conserved (8). Rev3p is a member of the B-family of DNA polymerases, which include polymerases alpha, delta, and epilson (4). Rev7p contains a HORMA domain that is found in proteins involved in cell-cycle control, meiosis, and DNA repair (20).

Last updated: 2010-02-02 Contact SGD

References cited on this page View Complete Literature Guide for REV3
1) Lemontt JF  (1971) Mutants of yeast defective in mutation induced by ultraviolet light. Genetics 68(1):21-33
2) Cassier-Chauvat C and Moustacchi E  (1988) Allelism between pso1-1 and rev3-1 mutants and between pso2-1 and snm1 mutants in Saccharomyces cerevisiae. Curr Genet 13(1):37-40
3) Henriques JA and Moustacchi E  (1980) Isolation and characterization of pso mutants sensitive to photo-addition of psoralen derivatives in Saccharomyces cerevisiae. Genetics 95(2):273-88
4) Prakash S, et al.  (2005) Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function. Annu Rev Biochem 74:317-53
5) Kolas NK and Durocher D  (2006) DNA repair: DNA polymerase zeta and Rev1 break in. Curr Biol 16(8):R296-9
6) Lee KY and Myung K  (2008) PCNA Modifications for Regulation of Post-Replication Repair Pathways. Mol Cells 26(1):5-11
7) Makarova AV, et al.  (2012) A four-subunit DNA polymerase ? complex containing Pol d accessory subunits is essential for PCNA-mediated mutagenesis. Nucleic Acids Res 40(22):11618-26
8) Gan GN, et al.  (2008) DNA polymerase zeta (pol zeta) in higher eukaryotes. Cell Res 18(1):174-83
9) Nelson JR, et al.  (1996) Thymine-thymine dimer bypass by yeast DNA polymerase zeta. Science 272(5268):1646-9
10) Hirano Y and Sugimoto K  (2006) ATR homolog Mec1 controls association of DNA polymerase zeta-Rev1 complex with regions near a double-strand break. Curr Biol 16(6):586-90
11) Johnson RE, et al.  (2000) Eukaryotic polymerases iota and zeta act sequentially to bypass DNA lesions. Nature 406(6799):1015-9
12) Guo D, et al.  (2001) Translesion synthesis by yeast DNA polymerase zeta from templates containing lesions of ultraviolet radiation and acetylaminofluorene. Nucleic Acids Res 29(13):2875-83
13) Haracska L, et al.  (2003) Yeast DNA polymerase zeta is an efficient extender of primer ends opposite from 7,8-dihydro-8-Oxoguanine and O6-methylguanine. Mol Cell Biol 23(4):1453-9
14) Haracska L, et al.  (2001) Roles of yeast DNA polymerases delta and zeta and of Rev1 in the bypass of abasic sites. Genes Dev 15(8):945-54
15) Johnson RE, et al.  (2001) Role of DNA polymerase eta in the bypass of a (6-4) TT photoproduct. Mol Cell Biol 21(10):3558-63
16) Johnson RE, et al.  (2003) Yeast DNA polymerase zeta (zeta) is essential for error-free replication past thymine glycol. Genes Dev 17(1):77-87
17) Lawrence CW, et al.  (1985) UV and chemical mutagenesis in rev7 mutants of yeast. Mol Gen Genet 200(1):86-91
18) Johnson RE, et al.  (1998) Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites. Genes Dev 12(19):3137-43
19) Holbeck SL and Strathern JN  (1997) A role for REV3 in mutagenesis during double-strand break repair in Saccharomyces cerevisiae. Genetics 147(3):1017-24
20) Aravind L and Koonin EV  (1998) The HORMA domain: a common structural denominator in mitotic checkpoints, chromosome synapsis and DNA repair. Trends Biochem Sci 23(8):284-6