MLH3/YPL164C Summary Help

Standard Name MLH3
Systematic Name YPL164C
Feature Type ORF, Verified
Description Protein involved in DNA mismatch repair and meiotic recombination; involved in crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability (1, 2, 3, 4 and see Summary Paragraph)
Chromosomal Location
ChrXVI:241497 to 239350 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MLH3 GO evidence and references
  View Computational GO annotations for MLH3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
21 total interaction(s) for 10 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-Western: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 6

Genetic Interactions
  • Negative Genetic: 1
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 715
Molecular Weight (Da) 82,000
Isoelectric Point (pI) 9.31
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:241497 to 239350 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2148 241497..239350 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006085

MLH3, one of four MutL homologs in S. cerevisiae, is involved in mismatch repair during mitosis and meiosis and plays a role in promoting crossing-over during meiotic recombination that is separate from its mismatch repair functions (reviewed in 5, 6). Like Pms1p and Mlh2p, two other MutL homologs, Mlh3p forms a heterodimer with Mlh1p, the fourth MutL homolog (7, 8, 2, 3). The Mlh1p-Mlh3p heterodimer is involved in the repair of specific types of mismatches (2, 1, 9) but its role in general mismatch repair is limited. An mlh3 mutant displays a reduced level of crossing-over during meiosis similar to an mlh1 single mutant, an msh4 single mutant, and an mlh1 msh4 double mutant, suggesting that the Mlh1p-Mlh3p heterodimer may interact with the Msh4p-Msh5p heterodimer to regulate cross-overs (10, 3). In addition, the Mlh1p-Mlh3p heterodimer may act to resolve cross-overs by interacting with the Sgs1p-Top3p heterodimer (11).

Last updated: 2007-05-29 Contact SGD

References cited on this page View Complete Literature Guide for MLH3
1) Harfe BD, et al.  (2000) Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast. Curr Biol 10(3):145-8
2) Flores-Rozas H and Kolodner RD  (1998) The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations. Proc Natl Acad Sci U S A 95(21):12404-9
3) Wang TF, et al.  (1999) Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction. Proc Natl Acad Sci U S A 96(24):13914-9
4) Lipkin SM, et al.  (2000) MLH3: a DNA mismatch repair gene associated with mammalian microsatellite instability. Nat Genet 24(1):27-35
5) Kolodner RD and Marsischky GT  (1999) Eukaryotic DNA mismatch repair. Curr Opin Genet Dev 9(1):89-96
6) Borts RH, et al.  (2000) The many faces of mismatch repair in meiosis. Mutat Res 451(1-2):129-50
7) Prolla TA, et al.  (1994) MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science 265(5175):1091-3
8) Pang Q, et al.  (1997) Functional domains of the Saccharomyces cerevisiae Mlh1p and Pms1p DNA mismatch repair proteins and their relevance to human hereditary nonpolyposis colorectal cancer-associated mutations. Mol Cell Biol 17(8):4465-73
9) Stone JE and Petes TD  (2006) Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae. Genetics 173(3):1223-39
10) Hunter N and Borts RH  (1997) Mlh1 is unique among mismatch repair proteins in its ability to promote crossing-over during meiosis. Genes Dev 11(12):1573-82
11) Wang TF and Kung WM  (2002) Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells. Biochem Biophys Res Commun 296(4):949-53