KES1/YPL145C Summary

KES1/YPL145C Summary

Standard Name	KES1
Systematic Name	YPL145C
Alias	LPI3 , OSH4 1 , BSR3 2 , 3
Feature Type	ORF, Verified
Description	One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication (3, 4, 5)
Name Description	KrE11-1 Suppressor 6

Chromosomal Location	ChrXVI:279699 to 278395 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All KES1 GO evidence and references

	View Computational GO annotations for KES1
Molecular Function
Manually curated	lipid binding (IDA, IMP) oxysterol binding (IMP, IPI, ISS) phosphatidic acid binding (IDA) phosphatidylinositol-4,5-bisphosphate binding (IMP, IPI) phosphatidylinositol-4-phosphate binding (IDA) sterol transporter activity (IDA, IMP)
Biological Process
Manually curated	endocytosis (IGI) exocytosis (IGI) maintenance of cell polarity (IGI) positive regulation of phosphatase activity (IDA) post-Golgi vesicle-mediated transport (IGI) sterol transport (IDA, IGI, IMP)
Cellular Component
Manually curated	cytoplasm (IDA) extrinsic to membrane (IDA) Golgi membrane (IDA)
High-throughput	cytoplasm (IDA)

Mutant phenotypes All KES1 Phenotype evidence and references

Classical genetics
null	nutrient uptake: decreased Fus-Mid-GFP distribution: abnormal resistance to nystatin: increased vegetative growth: decreased vegetative growth: normal viable
Large-scale survey
null	competitive fitness: decreased endocytosis: decreased Fus-Mid-GFP distribution: abnormal resistance to mefloquine: decreased resistance to wortmannin: normal resistance to sirolimus: decreased resistance to cycloheximide: decreased starvation resistance: decreased vacuolar morphology: abnormal viable
overexpression	cell cycle progression: abnormal vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All KES1 Interaction evidence and references

	229 total interaction(s) for 167 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-Western: 8 Biochemical Activity: 1 PCA: 12
Genetic Interactions	Dosage Growth Defect: 9 Dosage Lethality: 4 Dosage Rescue: 2 Negative Genetic: 122 Phenotypic Enhancement: 6 Phenotypic Suppression: 4 Positive Genetic: 6 Synthetic Growth Defect: 10 Synthetic Lethality: 16 Synthetic Rescue: 18
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All KES1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:279699 to 278395 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1305	279699..278395	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000006066

References cited on this page View Complete Literature Guide for KES1

1)	Beh CT, et al. (2001) Overlapping functions of the yeast oxysterol-binding protein homologues. Genetics 157(3):1117-40
2)	Cleves AE, et al. (1991) Mutations in the CDP-choline pathway for phospholipid biosynthesis bypass the requirement for an essential phospholipid transfer protein. Cell 64(4):789-800
3)	Fang M, et al. (1996) Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis. EMBO J 15(23):6447-59
4)	Li X, et al. (2002) Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex. J Cell Biol 157(1):63-77
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Jiang B, et al. (1994) A new family of yeast genes implicated in ergosterol synthesis is related to the human oxysterol binding protein. Yeast 10(3):341-53