BEM3/YPL115C Summary

BEM3 BASIC INFORMATION

Standard Name	BEM3 1, 2
Systematic Name	YPL115C
Feature Type	ORF, Verified
Description	Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly (1)
Name Description	Bud EMergence 3

GO Annotations	All BEM3 GO evidence and references
	View Computational GO annotations for BEM3
Molecular Function
Manually curated	phosphatidylinositol 3-phosphate binding (IDA) Rho GTPase activator activity (IDA, IGI, IPI)
Biological Process
Manually curated	budding cell apical bud growth (IPI) budding cell isotropic bud growth (IPI) establishment of cell polarity (TAS) invasive growth in response to glucose limitation (IPI) negative regulation of Rho protein signal transduction (IGI, IMP, IPI) pseudohyphal growth (IPI)
Cellular Component
Manually curated	cell cortex (IDA) cellular bud tip (IDA) cytoplasm (IDA) incipient cellular bud site (IDA) mating projection tip (IDA)
High-throughput	mating projection tip (IDA)

Mutant Phenotype	All BEM3 Phenotype details and references
Classical genetics
null	actin cytoskeleton morphology: abnormal bud morphology: abnormal cellular morphology: abnormal Scs2deltaTMD-GFP distribution: abnormal Kss1p modification: increased shmoo formation: abnormal
overexpression	actin cytoskeleton morphology: abnormal bud morphology: abnormal inviable shmoo formation: decreased viable
unspecified	viability: decreased
Large-scale survey
null	UV resistance: decreased resistance to caffeine: decreased viable

Interactions	BEM3 All interactions details and references
	60 total interaction(s) for 48 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 Biochemical Activity: 2 Protein-peptide: 1 Two-hybrid: 13
Genetic Interactions	Dosage Rescue: 7 Phenotypic Enhancement: 15 Phenotypic Suppression: 3 Synthetic Growth Defect: 5 Synthetic Lethality: 4 Synthetic Rescue: 2

Sequence Information

ChrXVI:335485 to 332099 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3387	335485..332099	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000006036

BEM3 RESOURCES

Click on map for expanded view

SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for BEM3

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BEM3]

1)	Zheng Y, et al. (1994) Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3. J Biol Chem 269(4):2369-72
2)	Zheng Y, et al. (1993) Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. J Biol Chem 268(33):24629-34
3)	Bender A and Pringle JR (1991) Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(3):1295-305

SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.