ELP4/YPL101W Summary Help

Standard Name ELP4 1, 2
Systematic Name YPL101W
Alias HAP1 , TOT7 3 , KTI9 4
Feature Type ORF, Verified
Description Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity (5, 6, 7, 8 and see Summary Paragraph)
Name Description ELongator Protein 1
Chromosomal Location
ChrXVI:360209 to 361579 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ELP4 GO evidence and references
  View Computational GO annotations for ELP4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
365 total interaction(s) for 218 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 26
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Co-crystal Structure: 2
  • Co-purification: 4
  • PCA: 8
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 10
  • Negative Genetic: 188
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 1
  • Positive Genetic: 29
  • Synthetic Growth Defect: 52
  • Synthetic Lethality: 25
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 456
Molecular Weight (Da) 51,155
Isoelectric Point (pI) 10.27
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:360209 to 361579 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1371 360209..361579 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006022
SUMMARY PARAGRAPH for ELP4

Elp4p is part of the six-subunit Elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II holoenzyme responsible for transcriptional elongation (9, 10, 3). Elongator contains two discrete subcomplexes, one consisting of Iki3p/Elp1p, Elp2p, and Elp3p, and the other consisting of Elp4p, Iki1p/Elp5p, and Elp6p (1, 2). Elongator binds to both naked and nucleosomal DNA, can acetylate both core histones and nucleosomal substrates, and plays a role in chromatin remodeling (11, 5). Its activity is directed specifically toward the amino-terminal tails of histone H3 and H4, with the predominant acetylation sites being lysine-14 of histone H3 and lysine-8 of histone H4 (5).

Of the six Elongator subunits, only Iki1p/Elp5p is essential for growth, and deletion of the other individual subunits causes significantly altered mRNA expression levels for many genes (2). Disruption of the Elongator complex confers resistance to the Kluyveromyces lactis zymotoxin (6), and a reduced sensitivity to the Pichia inositovora toxin (12).

Last updated: 2004-05-12 Contact SGD

References cited on this page View Complete Literature Guide for ELP4
1) Winkler GS, et al.  (2001) RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes. J Biol Chem 276(35):32743-9
2) Krogan NJ and Greenblatt JF  (2001) Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae. Mol Cell Biol 21(23):8203-12
3) Jablonowski D, et al.  (2001) Kluyveromyces lactis zymocin mode of action is linked to RNA polymerase II function via Elongator. Mol Microbiol 42(4):1095-105
4) Butler AR, et al.  (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14(9):6306-16
5) Winkler GS, et al.  (2002) Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo. Proc Natl Acad Sci U S A 99(6):3517-22
6) Frohloff F, et al.  (2003) Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex. J Biol Chem 278(2):956-61
7) Esberg A, et al.  (2006) Elevated levels of two tRNA species bypass the requirement for elongator complex in transcription and exocytosis. Mol Cell 24(1):139-48
8) Glatt S, et al.  (2012) The Elongator subcomplex Elp456 is a hexameric RecA-like ATPase.LID - 10.1038/nsmb.2234 [doi] Nat Struct Mol Biol ()
9) Wittschieben BO, et al.  (1999) A novel histone acetyltransferase is an integral subunit of elongating RNA polymerase II holoenzyme. Mol Cell 4(1):123-8
10) Frohloff F, et al.  (2001) Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin. EMBO J 20(8):1993-2003
11) Wittschieben BO, et al.  (2000) Overlapping roles for the histone acetyltransferase activities of SAGA and elongator in vivo. EMBO J 19(12):3060-8
12) Klassen R and Meinhardt F  (2003) Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora. Mol Genet Genomics 270(2):190-9