SSU1/YPL092W Summary Help

Standard Name SSU1
Systematic Name YPL092W
Alias LPG16
Feature Type ORF, Verified
Description Plasma membrane sulfite pump involved in sulfite metabolism; required for efficient sulfite efflux; major facilitator superfamily protein (1, 2 and see Summary Paragraph)
Chromosomal Location
ChrXVI:373793 to 375169 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SSU1 GO evidence and references
  View Computational GO annotations for SSU1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 125 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
13 total interaction(s) for 13 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • PCA: 3
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 3
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 458
Molecular Weight (Da) 52,545
Isoelectric Point (pI) 8.54
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:373793 to 375169 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1377 373793..375169 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006013
SUMMARY PARAGRAPH for SSU1

SSU1 encodes a plasma membrane sulfite pump involved in sulfite metabolism (1) and required for efficient sulfite efflux (2). Ssu1p lacks the nucleotide binding sequence typical of ABC transporters, but resembles the general structure of facilitator/transporter proteins (3). Ssu1p is a member of the major facilitator superfamily involved in efflux of toxic compounds, specifically mediating efflux of the free form of sulfite (2). Ssu1p has no yeast homologs and is the sole identified target of the Fzf1p transcriptional activator (2). Mutations in SSU1 cause sulfite sensitivity (4). Overexpression confers heightened sulfite resistance (5, 6) and suppresses sensitivity of unrelated sulfite mutants, indicating that sulfite efflux mediated by Ssu1p is a major detoxification pathway (2).

Last updated: 2005-02-18 Contact SGD

References cited on this page View Complete Literature Guide for SSU1
1) Avram D and Bakalinsky AT  (1997) SSU1 encodes a plasma membrane protein with a central role in a network of proteins conferring sulfite tolerance in Saccharomyces cerevisiae. J Bacteriol 179(18):5971-4
2) Park H and Bakalinsky AT  (2000) SSU1 mediates sulphite efflux in Saccharomyces cerevisiae. Yeast 16(10):881-8
3) Balzi E and Goffeau A  (1994) Genetics and biochemistry of yeast multidrug resistance. Biochim Biophys Acta 1187(2):152-62
4) Xu X, et al.  (1994) Isolation and characterization of sulfite mutants of Saccharomyces cerevisiae. Curr Genet 25(6):488-96
5) Park H, et al.  (1999) Use of sulfite resistance in Saccharomyces cerevisiae as a dominant selectable marker. Curr Genet 36(6):339-44
6) Goto-Yamamoto N, et al.  (1998) SSU1-R, a sulfite resistance gene of wine yeast, is an allele of SSU1 with a different upstream sequence. J Ferment Bioeng 86:427-433