BRO1/YPL084W Summary Help

Standard Name BRO1 1
Systematic Name YPL084W
Alias LPF2 , VPS31 2 , 3 , ASI6 , NPI3
Feature Type ORF, Verified
Description Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes (4, 5 and see Summary Paragraph)
Name Description BCK1-like Resistance to Osmotic shock 1
Chromosomal Location
ChrXVI:394038 to 396572 | ORF Map | GBrowse
Gene Ontology Annotations All BRO1 GO evidence and references
  View Computational GO annotations for BRO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
124 total interaction(s) for 92 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-Western: 12
  • Co-localization: 2
  • Co-purification: 1
  • PCA: 5
  • Reconstituted Complex: 2
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 28
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 5
  • Positive Genetic: 6
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 11
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 844
Molecular Weight (Da) 97,275
Isoelectric Point (pI) 5.15
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:394038 to 396572 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2535 394038..396572 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006005

Bro1p is a cytoplasmic class E vacuolar protein sorting (VPS) factor that associates with endosomal compartments and functions in the multivesicular body (MVB) pathway (4). The endosomal association of Bro1p is specifically dependent on Snf7p, whereas its dissociation from endosomes requires Vps4p, a class E VPS ATPase that regulates the endosomal dissociation of ESCRT-III, a complex of four class E VPS proteins (Did4p, Vps20p, Vps24p and Snf7p) that oligomerize at the endosomal membrane (4). Bro1p coordinates the timing and location of deubiquitination in the multivesicular body pathway by recruiting Doa4p, the ubiquitin thiolesterase required for the removal of ubiquitin from MVB cargoes, to endosomes. Loss of Bro1p is suppressed by the overexpression of DOA4, which restores cargo protein deubiquitination and sorting via the MVB pathway and reverses the abnormal endosomal morphology typical of bro1 mutant cells, resulting in the restoration of multivesicular endosomes (5).

Last updated: 2004-10-21 Contact SGD

References cited on this page View Complete Literature Guide for BRO1
1) Nickas ME and Yaffe MP  (1996) BRO1, a novel gene that interacts with components of the Pkc1p-mitogen-activated protein kinase pathway in Saccharomyces cerevisiae. Mol Cell Biol 16(6):2585-93
2) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
3) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4) Odorizzi G, et al.  (2003) Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae. J Cell Sci 116(Pt 10):1893-903
5) Luhtala N and Odorizzi G  (2004) Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes. J Cell Biol 166(5):717-29