MUK1/YPL070W Summary Help

Standard Name MUK1 1
Systematic Name YPL070W
Feature Type ORF, Verified
Description Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF VPS9; required for localization of the CORVET complex to endosomes; contains a VPS9 domain (2, 3, 4, 5)
Name Description coMpUtationally-linked to Kap95 4
Chromosomal Location
ChrXVI:420948 to 422786 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MUK1 GO evidence and references
  View Computational GO annotations for MUK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
Resources
64 total interaction(s) for 54 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • PCA: 1
  • Two-hybrid: 43

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 3
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 612
Molecular Weight (Da) 69,538
Isoelectric Point (pI) 8.8
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:420948 to 422786 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1839 420948..422786 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005991
References cited on this page View Complete Literature Guide for MUK1
1) Samanta MP and Liang S  (2003) Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci U S A 100(22):12579-83
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3) Carney DS, et al.  (2006) Vps9 domain-containing proteins: activators of Rab5 GTPases from yeast to neurons. Trends Cell Biol 16(1):27-35
4) Paulsel AL, et al.  (2013) Vps9 family protein muk1 is the second rab5 guanosine nucleotide exchange factor in budding yeast. J Biol Chem 288(25):18162-71
5) Cabrera M, et al.  (2013) Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis. J Biol Chem 288(7):5166-75