VPS28/YPL065W Summary

VPS28/YPL065W Summary

Standard Name	VPS28 1, 2
Systematic Name	YPL065W
Alias	VPT28 1 , VPL13 2
Feature Type	ORF, Verified
Description	Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p (3, 4)
Name Description	Vacuolar Protein Sorting 1, 2

Chromosomal Location	ChrXVI:427927 to 428655 \| ORF Map \| GBrowse

Gene Ontology Annotations All VPS28 GO evidence and references

	View Computational GO annotations for VPS28
Molecular Function
Manually curated	protein complex binding (IDA, IPI)
Biological Process
Manually curated	protein targeting to membrane (IMP, IPI) protein targeting to vacuole (IMP, IPI) ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway (IC)
Cellular Component
Manually curated	endosome (IDA) ESCRT I complex (IDA, IPI)

Mutant phenotypes All VPS28 Phenotype evidence and references

Classical genetics
null	biofilm formation: decreased invasive growth: absent metal resistance: decreased metal resistance: increased Ptr2p-GFP distribution: abnormal carboxypeptidase Y (Prc1p) modification: absent Pep4p modification: absent resistance to sulfometuron: decreased toxin resistance: increased toxin resistance: decreased vacuolar morphology: abnormal
reduction of function	endomembrane system morphology: abnormal vacuolar transport: abnormal
Large-scale survey
null	acid pH resistance: decreased auxotrophy glycogen accumulation: decreased inositol excretion: increased glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased hyperosmotic stress resistance: decreased ionic stress resistance: decreased oxidative stress resistance: decreased resistance to lovastatin: decreased resistance to BPS: decreased resistance to clioquinol: decreased resistance to mefloquine: decreased resistance to dimethyl sulfoxide: decreased resistance to boric acid: decreased resistance to neothyonidioside: decreased resistance to methyl methanesulfonate: decreased resistance to caffeine: decreased resistance to tirapazamine: decreased resistance to ethanol: decreased resistance to doxorubicin: decreased resistance to sirolimus: decreased resistance to nickel sulfate: increased resistance to wortmannin: increased resistance to cycloheximide: decreased resistance to neomycin: decreased resistance to pyocyanin: decreased resistance to sorbate: decreased resistance to hygromycin B: decreased resistance to calcium dichloride: decreased respiratory growth: decreased rate sporulation efficiency: decreased stress resistance: increased telomere length: decreased toxin resistance: decreased transposable element transposition: decreased utilization of nitrogen source: absent vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	filamentous growth: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All VPS28 Interaction evidence and references

	108 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 2 Affinity Capture-Western: 8 Co-crystal Structure: 2 Co-fractionation: 2 Co-purification: 3 Reconstituted Complex: 1 Two-hybrid: 11
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 18 Phenotypic Enhancement: 16 Phenotypic Suppression: 27 Positive Genetic: 3 Synthetic Growth Defect: 4 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 2 Synthetic Rescue: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All VPS28 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:427927 to 428655 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..729	427927..428655	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005986

References cited on this page View Complete Literature Guide for VPS28

1)	Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2)	Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3)	Katzmann DJ, et al. (2001) Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell 106(2):145-55
4)	Pineda-Molina E, et al. (2006) The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment. Traffic 7(8):1007-16