ERG10/YPL028W Summary Help

Standard Name ERG10 1, 2
Systematic Name YPL028W
Alias LPB3 , TSM0115
Feature Type ORF, Verified
Description Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase); cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis (1 and see Summary Paragraph)
Name Description ERGosterol biosynthesis 1, 2
Chromosomal Location
ChrXVI:498096 to 499292 | ORF Map | GBrowse
Genetic position: -22 cM
Gene Ontology Annotations All ERG10 GO evidence and references
  View Computational GO annotations for ERG10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Classical genetics
Large-scale survey
reduction of function
106 total interaction(s) for 93 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 6
  • Co-purification: 1
  • PCA: 2

Genetic Interactions
  • Negative Genetic: 70
  • Positive Genetic: 6
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 398
Molecular Weight (Da) 41,728
Isoelectric Point (pI) 7.38
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:498096 to 499292 | ORF Map | GBrowse
Genetic position: -22 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1197 498096..499292 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005949

ERG10 encodes acetyl-CoA C-acetyltransferase (also called acetoacetyl-CoA thiolase), a cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA (1, 3). The formation of acetoacetyl-CoA is the first step in the biosynthesis of mevalonate, which is required in turn for the biosynthesis of sterols and nonsterol isoprenoids (3); in yeast, ergosterol is the end product of the sterol biosynthetic pathway (4, 5).

Cells lacking Erg10p are viable but lack detectable acetyl-CoA C-acetyltransferase and are mevalonate auxotrophs (1). Expression of ERG10 is regulated by a sterol-mediated feedback system (6).

Enzymes similar to Erg10p are found in many diverse organisms (1); the enzymes from Saccharomyces uvarum and radish can complement S. cerevisiae erg10 mutants (7, 8).

Last updated: 2000-08-21 Contact SGD

References cited on this page View Complete Literature Guide for ERG10
1) Hiser L, et al.  (1994) ERG10 from Saccharomyces cerevisiae encodes acetoacetyl-CoA thiolase. J Biol Chem 269(50):31383-9
2) Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77
3) Stryer L  (1995) Biochemistry (4th ed.). New York: W. H. Freeman and Company
4) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
6) Dimster-Denk D and Rine J  (1996) Transcriptional regulation of a sterol-biosynthetic enzyme by sterol levels in Saccharomyces cerevisiae. Mol Cell Biol 16(8):3981-9
7) Dequin S, et al.  (1988) Cloning, sequencing and analysis of the yeast S. uvarum ERG10 gene encoding acetoacetyl CoA thiolase. Curr Genet 13(6):471-8
8) Vollack KU and Bach TJ  (1996) Cloning of a cDNA encoding cytosolic acetoacetyl-coenzyme A thiolase from radish by functional expression in Saccharomyces cerevisiae. Plant Physiol 111(4):1097-107