RMI1/YPL024W Summary

RMI1 BASIC INFORMATION

Standard Name	RMI1 1, 2
Systematic Name	YPL024W
Alias	NCE4
Feature Type	ORF, Verified
Description	Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing and ssDNA binding activities of Top3p; involved in response to DNA damage; null mutants display increased rates of recombination and delayed S phase (1, 3)
Name Description	RecQ Mediated genome Instability 1, 2

GO Annotations	All RMI1 GO evidence and references
	View Computational GO annotations for RMI1
Molecular Function
Manually curated	enzyme activator activity (IDA) four-way junction DNA binding (IDA) single-stranded DNA binding (IDA)
Biological Process
Manually curated	mitotic sister chromatid cohesion (IGI, IMP) positive regulation of DNA binding (IDA) response to DNA damage stimulus (IGI)
Cellular Component
Manually curated	RecQ helicase-Topo III complex (IDA, IPI)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant Phenotype	All RMI1 Phenotype details and references
Classical genetics
null	cell cycle progression through the G2/M phase transition: delayed cellular morphology: abnormal chromosome/plasmid maintenance: abnormal colony sectoring: increased mutation frequency: increased resistance to benomyl: decreased vegetative growth: decreased
Large-scale survey
null	competitive fitness: decreased filamentous growth: decreased invasive growth: absent mitotic recombination: increased Rad52-YFP distribution: increased resistance to hydroxyurea: decreased resistance to 5-fluorouracil: decreased resistance to methyl methanesulfonate: decreased resistance to doxorubicin: decreased respiratory growth: decreased rate transposable element transposition: increased viable

Interactions	RMI1 All interactions details and references
	150 total interaction(s) for 104 unique genes/features.
Physical Interactions	Affinity Capture-Western: 7 Biochemical Activity: 1 Co-fractionation: 3 Co-purification: 1 Reconstituted Complex: 2
Genetic Interactions	Phenotypic Enhancement: 82 Phenotypic Suppression: 8 Synthetic Growth Defect: 5 Synthetic Lethality: 32 Synthetic Rescue: 9

Sequence Information

ChrXVI:503515 to 504240 | |

Last Update

Coordinates: 2004-07-21 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..726	503515..504240	2004-07-21	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005945

RMI1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for RMI1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RMI1]

1)	Chang M, et al. (2005) RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. EMBO J 24(11):2024-33
2)	Mullen JR, et al. (2005) Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Mol Cell Biol 25(11):4476-87
3)	Chen CF and Brill SJ (2007) Binding and activation of DNA topoisomerase III by the Rmi1 subunit. J Biol Chem 282(39):28971-9

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