CTF19/YPL018W Summary Help

Standard Name CTF19 1
Systematic Name YPL018W
Alias MCM18
Feature Type ORF, Verified
Description Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 (1, 2, 3, 4, 5)
Name Description Chromosome Transmission Fidelity 6
Chromosomal Location
ChrXVI:517651 to 518760 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CTF19 GO evidence and references
  View Computational GO annotations for CTF19
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
334 total interaction(s) for 194 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Co-localization: 4
  • PCA: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 101

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 7
  • Dosage Rescue: 1
  • Negative Genetic: 76
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Positive Genetic: 3
  • Synthetic Growth Defect: 36
  • Synthetic Lethality: 45
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 369
Molecular Weight (Da) 42,782
Isoelectric Point (pI) 8.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:517651 to 518760 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1110 517651..518760 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005939
References cited on this page View Complete Literature Guide for CTF19
1) Hyland KM, et al.  (1999) Ctf19p: A novel kinetochore protein in Saccharomyces cerevisiae and a potential link between the kinetochore and mitotic spindle. J Cell Biol 145(1):15-28
2) Ortiz J, et al.  (1999) A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev 13(9):1140-55
3) De Wulf P, et al.  (2003) Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev 17(23):2902-21
4) Schleiffer A, et al.  (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13
5) Matson DR, et al.  (2012) A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. Genes Dev 26(6):542-7
6) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49